≥97% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied, They can be ordered from .Golgi-Tracker Green is suitable for staining Golgi in live cells, but not Golgi in fixed cells.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
Instructions for Use：
1.Preparation of Golgi-Tracker Green working solution a.Dilute an appropriate amount of Golgi-Tracker Green (33.3mg/ml) in the Golgi-Tracker Red Dilution Buffer at a ratio of 1:100. For example, mix 1µl of Golgi-Tracker Green with 1ml of Golgi-Tracker Green Dilution Buffer to obtain the Golgi-Tracker Green working solution.Note: The dilution ratio of Golgi-Tracker Green can be adjusted within the range of 1:50-1:200 based on the staining results.b.Golgi-Tracker Green working solution can be recovered and stored at 4℃ or -20℃ for future uses until it no longer works. Store at 4℃ if it will be reused within 1-2 days , or at -20℃ for longer period of storage.2.Fluorescent labeling of Golgi bodies in live cellsa.Remove the cell culture medium and wash the cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells.b.Remove the wash buffer, add the Golgi-Tracker Green staining working solution prepared in step 1 and incubate at 4℃ for 30 min.c.Recover the Golgi-Tracker Green staining working solution, wash the cells about 3 times with cell culture medium pre-cooled on ice, and incubate with fresh culture medium at 37℃ for 30 minutes.d.Wash again with fresh culture medium, followed by observation under fluorescence microscope or confocal microscope. Bright green fluorescent Golgi bodies can be observed, while other membrane systems within cells show a fainter fluorescent staining.
Excitation(nm): Blue Laser (488 nm)
Emission(nm): 512nm
Ex/Em(nm): Ex:505nm;Em:512nm
| Peso molecular | 601.62 |
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Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Sensibilidad | light sensitive |
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H2521409