Microfilaments are mainly formed by the polymerization of actin and are an important component of the cytoskeleton. They participate in the maintenance of cell morphology, adhesion and migration, cytokinesis, endocytic transport, stress fiber formation, and the establishment of cell polarity.
Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...
Mounting is not merely an ancillary step after staining, but a critical determinant of section transparency, fluorescence stability, background control, and storage life. In experiments such as immunofluorescence, immunohistochemistry, in situ hybridization, and lipid staining, different ...
Antibody fluorescent labeling introduces fluorophores or reporter systems into antibody molecules via covalent conjugation or other stable linkage strategies, enabling target antigens to yield imageable and quantitatively measurable signals. It is a foundational technology for platforms such as ...
Immunofluorescence Protocol
Multiplexed immunohistochemistry (mIHC) is an in situ multiple labeling method based on tyramide signal amplification (TSA) technology, which can achieve simultaneous detection of multiple targets (usually 6-8 types) on a single tissue section. Its core principle is to covalently bind the target ...
Immunohistochemistry FAQ Analysis
In cell biology research, staining techniques are essential tools for observing the internal structures and functions of cells. The endoplasmic reticulum (ER) and other organelles such as mitochondria, Golgi apparatus, and lysosomes play specific biological roles.
Guide to immunofluorescence experiments
