GRADE & PURITY Validated, ExactAb™, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

W6/32

Conjugation

AF488

Descripción general

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for Flow Cytometry.

Specifications

Product Name

HLA Class I Mouse mAb (AF488) - Primary antibody, specific to HLA Class I

Sinónimos

A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo

Especificaciones y pureza

ExactAb™, Validated, 5 μL/test

Especies hospedadoras

Mouse

Especificidad

HLA Class I

Inmunógeno

Human tonsil cell membrane

Control positivo

Flow: Human peripheral blood lymphocytes

Conjugación

AF488

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

5 μL/test

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: HLA Class I Mouse mAb (AF488) (Ab191628)

HLA Class I Mouse mAb (AF488) (Ab191628) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with HLA Class I Mouse mAb (AF488) (Ab191628) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

Flow Cytometry: HLA Class I Mouse mAb (AF488) (Ab191628)

HLA Class I Mouse mAb (AF488) (Ab191628) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with HLA Class I Mouse mAb (AF488) (Ab191628) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Objetivos asociados (humanos)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)