Hyaluronidase from bovine testes, CAS No.37326-33-3

CAS: 37326-33-3 Cat. No.: H754935 Número EC: 253-464-3
Disponible para pedir
GRADE & PURITY Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Native ? Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required. Endotoxin tested ? Endotoxin-tested — each lot assayed and labeled with its endotoxin level. Use when you need a documented endotoxin value for risk assessment. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. 400-1000 IU/mg enzyme powder
Accession #
Q5E985,Q8SQG8
Endotoxin Concentration
<0.2 EU/IU
Bioactivity
400-1000 IU/mg enzyme powder
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
100mg
H754935-100mg
1
84,90US$
250mg
H754935-250mg
1
135,90US$
1g
H754935-1g
1
323,90US$
5g
H754935-5g
1
1.289,90US$
Enter a quantity for the sizes you want to add.
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Why this grade

Bioactive,ActiBioPure™,Native,endotoxin tested,High Performance,EnzymoPure™,400-1000 IU/mg enzyme powder ActiBioPure™,Bioactive,Endotoxin tested,High Performance,Native,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 20 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.
Suitable for use in preparation of single cell suspension for sequencing.
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Specifications

Product Name
Hyaluronidase from bovine testes, CAS No.37326-33-3
Sinónimos
Hyaluronate 4-glycanohydrolase | Hyaluronoglucosaminidase | hyaluronoglucosaminidase | chondroitinase | chondroitinase I | hyaluronidase | hyaluronoglucosidase | Hyaluronate 4-glycanohydrolase | HYAL1 | HYAL2
Grado
ActiBioPure™, Bioactive, Endotoxin tested, High Performance, Native, EnzymoPure™
Especificaciones y pureza
Bioactive, ActiBioPure™, Native, endotoxin tested, High Performance, EnzymoPure™, 400-1000 IU/mg enzyme powder
Mecanismos bioquímicos y fisiológicos
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Bioactividad
400-1000 IU/mg enzyme powder
Concentración de endotoxinas
<0.2 EU/IU
Número de adhesión
CAS
37326-33-3
Número de la Comisión de Enzimas
3.2.1.35(BRENDA, IUBMB)
Tipo de molécula
Enzyme
Almacenamiento y envío
Concentración
400-1000 IU/mg enzyme powder
Condiciones de almacenamiento de almacenamiento
Store at -20°C
Enviado en
Ice chest + Ice pads
Definición de unidad
One unit (IU) of hyaluronidase is defined as the amount of enzyme required to hydrolyze hyaluronic acid to produce reducing sugars equivalent to 0.5 μg of glucuronic acid per minute at 37 ℃ and pH 5.3.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganoheterocyclic compounds
ClaseIndoles and derivatives
SubclassIndolyl carboxylic acids and derivatives
Intermediate Tree Nodes Not available
Direct ParentIndolyl carboxylic acids and derivatives
Alternative Parents Indoles  Tertiary alkylarylamines  Medium-chain fatty acids  Heterocyclic fatty acids  Amino fatty acids  Benzenoids  Amino acids  Monocarboxylic acids and derivatives  Enamines  Carboxylic acids  Azacyclic compounds  Organic oxides  Hydrocarbon derivatives  Hydrobromides  Carbonyl compounds  
Molecular FrameworkAromatic heteropolycyclic compounds
Substituents Indolyl carboxylic acid derivative - Indole - Medium-chain fatty acid - Tertiary aliphatic/aromatic amine - Amino fatty acid - Heterocyclic fatty acid - Fatty acyl - Benzenoid - Amino acid or derivatives - Amino acid - Tertiary amine - Carboxylic acid derivative - Carboxylic acid - Azacycle - Enamine - Monocarboxylic acid or derivatives - Amine - Organonitrogen compound - Organooxygen compound - Hydrobromide - Hydrocarbon derivative - Organic oxide - Carbonyl group - Organic oxygen compound - Organic nitrogen compound - Aromatic heteropolycyclic compound
DescripciónThis compound belongs to the class of organic compounds known as indolyl carboxylic acids and derivatives. These are compounds containing a carboxylic acid chain (of at least 2 carbon atoms) linked to an indole ring.
External Descriptors Not available
Estructura 3D
Modelo de Estructura Química Interactiva





Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

10 results found

Lot NumberCertificate TypeFechaArticulo
C2605541Certificate of AnalysisFeb 07, 2026 H754935
C2605542Certificate of AnalysisFeb 07, 2026 H754935
C2612780Certificate of AnalysisFeb 07, 2026 H754935
C2612781Certificate of AnalysisFeb 07, 2026 H754935
C2519052Certificate of AnalysisMar 31, 2025 H754935
C2519053Certificate of AnalysisMar 31, 2025 H754935
C2519054Certificate of AnalysisMar 31, 2025 H754935
C2519055Certificate of AnalysisMar 31, 2025 H754935
C2519056Certificate of AnalysisMar 31, 2025 H754935
L2510028Certificate of AnalysisMar 31, 2025 H754935
Preguntas frecuentes y artículos
Citations of This Product
Referencias
1. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong.  (2024)  Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing.  BIOMACROMOLECULES,      [PMID:38720431] [10.1021/acs.biomac.4c00261]
2. Mengyao Qi, Yi Ke, Yifei Li, Peng Li, Huakuo Zhou, Xia Zhang, Jieshi Chen, Jun Meng.  (2024)  Au@Pt nanozyme-based smart hydrogel for visual detection of hyaluronic acid.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.137144]
3. Tao Meng, Zhengyu Zhao, Mengqiang Song, Rui Wang, Xu Xu, Min Yang, Weihua Li, Shiyong Song.  (2025)  Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40090277] [10.1016/j.ijbiomac.2025.142155]
4. Yu Pang, Jie Lv, Chengcai He, Chengda Ju, Yulong Lin, Cong Zhang, Meng Li.  (2024)  Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:38330663] [10.1016/j.jcis.2024.01.217]
5. Siying Huang, Simeng Xiao, Xuehao Li, Ranran Tao, Zhangwei Yang, Ziwei Gao, Junjie Hu, Yan Meng, Guohua Zheng, Xinyan Chen.  (2024)  Development of Dual-Targeted Mixed Micelles Loaded with Celastrol and Evaluation on Triple-Negative Breast Cancer Therapy.  Pharmaceutics,  16  (9): (1174).  [PMID:39339211] [10.3390/pharmaceutics16091174]
6. Shiyi Yao, Hui Yuan, Luqi Yang, Yin Zhang, Hanyu Wang, Renxuan Li, Tingjun Ye, Wenguo Cui, Lei Wang.  (2025)  Downregulation of the PI3K/AKT/mTOR/MMP-13 pathway for promoting interface healing via lubricating microspheres.  Acta Biomaterialia,      [PMID:39761787] [10.1016/j.actbio.2025.01.001]
7. Jing Zhang, Yunfeng Zeng, Yongyuan Heng, Yu Shen, Ximeng Sun, Yijia Wang, Haiping Zheng, Ming Zeng, Ziyi Yu.  (2024)  Enzyme-Assisted Activation Technique for Producing Versatile Hydrogel Microparticle Scaffolds with High Surface Chemical Reactivity.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202400858]
8. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li.  (2024)  Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound.  ACS Nano,      [PMID:38949962] [10.1021/acsnano.4c02921]
9. Huiping Yang, Yinning Lin, Qian Mo, Zhaoquan Li, Fan Yang, Xinchun Li.  (2024)  Monitoring Enzymatic Reaction Kinetics and Activity Assays in Confined Nanospace.  ANALYTICAL CHEMISTRY,      [PMID:39024010] [10.1021/acs.analchem.4c01901]
10. Shuo Yu, Lu Zhang, Yanshen Yang, Meijuan Wang, Tingting Liu, Wenwen Ji, Yang Liu, Hao Lv, Yang Zhao, Xi Chen, Tinghua Hu.  (2024)  Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model.  ACS Pharmacology & Translational Science,      [PMID:38633596] [10.1021/acsptsci.3c00304]
11. Xin Jin, Chengxiong Wei, Kai Li, Peinan Yin, Chengwei Wu, Wei Zhang.  (2024)  Polyphenol-mediated hyaluronic acid/tannic acid hydrogel with short gelation time and high adhesion strength for accelerating wound healing.  CARBOHYDRATE POLYMERS,      [PMID:39048222] [10.1016/j.carbpol.2024.122372]
12. Dandan Wei, Guanhua Jiao, Yinghua Tao, Liuxin Yang, Tao Liu, Fengya Jing, Tianzhu Zhang.  (2025)  Polypropylene Mesh Coated with Dual Cross-Linked Hyaluronic Acid/Polyvinyl Alcohol Composite Hydrogel with Antiadhesion and Angiogenesis Properties for Abdominal Wall Repair.  Advanced Materials Technologies,      [PMID:] [10.1002/admt.202401786]
13. Dandan Wei, Yulin Huang, Min Liang, Liuxin Yang, Guanhua Jiao, Yinghua Tao, Li Xu, Tianzhu Zhang, Zhenling Ji.  (2024)  Polypropylene mesh coated with hyaluronic acid/polyvinyl alcohol composite hydrogel for preventing bowel adhesion.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38705326] [10.1016/j.ijbiomac.2024.132061]
14. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He.  (2025)  Printability in Multi-material Projection-Based 3-Dimensional Bioprinting.  Research,      [PMID:40041038] [10.34133/research.0613]
15. Zhu Dan, Zhong Hanyan, Shi Jingzhan, Liu Qiang, Wang Yiping.  (2025)  Ratiometric surface-enhanced Raman scattering quantification of extracellular matrix metalloproteinase-2 activity for tumor diagnosis.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,      [PMID:39966175] [10.1007/s00216-025-05792-5]
16. Yingying Chen, Dan Zhu, Hanyan Zhong, Zhixing Gan, Shenfei Zong, Zhuyuan Wang, Yiping Cui, Yiping Wang.  (2024)  Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure.  ACS Applied Materials & Interfaces,      [PMID:38204415] [10.1021/acsami.3c15344]
17. Shangting Du, Lingyu Li, Junhao Kou, Tianyi Zhu, Zhenyi Song, Yonghua Zhan, Daocheng Wu, Wenhua Zhan.  (2025)  Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy.  International Journal of Nanomedicine,      [PMID:40692537] [10.2147/IJN.S530041]
18. Jiancong Ni, Xiaochun Xu, Bifang Yang, Liyang Liu, Weiqiang Yang, Xiaoping Chen, Guiping Chen, Qingxiang Wang, Zhenyu Lin.  (2025)  Indicator-Free Detection of Hyaluronidase Based on a Conductivity-Regulated Bipolar Electrochemiluminescence Platform.  ANALYTICAL CHEMISTRY,      [PMID:40827572] [10.1021/acs.analchem.5c03409]
19. Haojie Qiu, Jingyan Zhu, Kaikai Zhang, Xinnan Wang, Yaoting Chu, Feng Zhang, Xuefeng Yang.  (2025)  Dual Drug-Loaded Hyaluronate-Based Self-Adaptable Hydrogel for Diabetic Infected Wound Healing.  BIOMACROMOLECULES,      [PMID:40845349] [10.1021/acs.biomac.5c00712]
20. Wenjun Xue, Xiaoyu Kuang, Xuanzhen Meng, Boyu Sun, Zijun Zhao, Guizhao Liang.  (2025)  Identification and inhibition mechanism of novel collagen-derived hyaluronidase inhibitory peptides.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.106263]
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