Proteinase K and Ribonuclease A

I. Proteinase K

1.Overview

Proteinase K is a broad-spectrum serine protease renowned for its exceptional stability and strong proteolytic activity. It retains high enzymatic activity under various denaturing conditions (e.g., in the presence of SDS or urea), making it particularly suitable for processing complex and rigid biological samples. Proteinase K efficiently degrades proteins while hydrolyzing peptide bonds without damaging DNA integrity, thereby releasing trapped nucleic acids and facilitating subsequent purification.

2.Applications

• Contaminant removal: Eliminates nucleases and other protein contaminants; widely used in DNA/RNA extraction kits to ensure sample purity.
• PCR sample preparation: Applied in pre-PCR treatment, especially in direct PCR, to simplify sample handling and improve efficiency.
• Viral nucleic acid detection: Disrupts viral capsids to expose nucleic acids for detection.
• Biopharmaceuticals: Utilized in the extraction of nucleic acids, polysaccharides, and other non-protein biomolecules; broadly applied in the production of DNA vaccines, heparin, and related products.

Figure 1: Chemical Structure of Proteinase K

3. Advantages of Aladdin Proteinase K:

• High stability and broad-spectrum activity: Proteinase K maintains activity under various pH levels, salt buffers, detergents (e.g., SDS), and temperature conditions. It efficiently hydrolyzes a wide range of proteins and nucleases without compromising DNA integrity.
• Versatile applicability: Suitable for DNA/RNA separation, endotoxin removal, tissue section processing, mitochondrial enzyme localization, prion-like sample digestion, and various other applications.

4.Product List

Name	ID	Grade & Purity
Proteinase K	P109033	EnzymoPure™, lyophilized powder，≥30 units/mg protein
Proteinase K	P274341	EnzymoPure™, BioReagent
Proteinase K from Tritirachium album	P301575	EnzymoPure™, ≥500 units/mL, buffered aqueous glycerol solution
Proteinase K from Tritirachium album	P755752	UltraBio™, Suitable for molecular biology, lyophilized powder, ≥30 units/mg protein
Proteinase K from Tritirachium album limber	P128666	EnzymoPure™, ≥20 units/mg dry weight

II. RNase A

1.Overview

RNase A is an enzyme that specifically hydrolyzes RNA molecules by cleaving the phosphodiester bonds at the 3′ end of pyrimidine residues. Due to its high efficiency and specificity, RNase A has become an essential tool in molecular biology experiments for obtaining high-quality DNA, particularly indispensable for removing RNA contamination.

Figure 2: Chemical Structure of RNase A

2.Applications

• RNA removal and DNA purification: Eliminates RNA contamination during genomic DNA or plasmid extraction, enhancing DNA purity. Suitable for experiments with high DNA quality requirements, such as PCR and sequencing.
• RNase protection assays: Widely used in studies of RNA structure and function, providing specific cleavage to generate data necessary for in-depth analysis.

3.Advantages of Aladdin RNase A:

• Efficient RNA removal: Capable of eliminating RNA from plasmid DNA, genomic DNA, and protein samples, ensuring smooth downstream experiments.
• Wide applicability and stability: Suitable for RNA sequence analysis, RNase protection assays, drug design, and other applications. Exhibits strong stability and retains activity even after boiling.

4.Product List

Name	ID	Grade & Purity
RNase A	R665518	EnzymoPure™, DNase free, Protease Free, sterile, ≥90%(SDS-PAGE), 10 mg/mL
RNase A	R665521	DNase free, Protease Free, sterile, EnzymoPure™, ≥90%(SDS-PAGE), 100 mg/mL
Ribonuclease A	R401532	EnzymoPure™, ≥ 60 Kunitz units/mg Lyophilized Powder
Ribonuclease A from bovine pancreas	R128622	EnzymoPure™, ≥2,500 units/mg dry weight
Ribonuclease A from bovine pancreas(Purified)	R128620	EnzymoPure™, ≥3,000 units/mg dry weight
Ribonuclease A from bovine pancreas	R754999	Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥100 KU/mg protein
Ribonuclease A from bovine pancreas	R755000	Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥60 Kunitz units/mg protein
Ribonuclease A from bovine pancreas	R104906	EnzymoPure™, ≥ 50 Kunitz units/mg
Ribonuclease B from bovine pancreas	R128623	EnzymoPure™, ≥1,000 units/mg dry weight
Ribonuclease A from bovine pancreas(Purified Solution)	R128621	EnzymoPure™, ≥3,000 units/mg protein
Ribonuclease A from bovine pancreas(DNase & Protease Free)	R128619	EnzymoPure™, ≥2,000 units/mg protein

Aladdin: https://www.aladdinsci.com/