GRADE & PURITYEx:565nm?Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:670nm?Fluorescence emission maximum at 670 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.See COA
Ex:565nm, Em:670nm, See COA Em:670nm,Ex:565nm for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
PE-Cy5 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye. The resonance energy transfer dye Cy5-Phycoerythrin (Cy5PE) is an extremely bright dye, primarily because of the very low autofluorescence in the region where it fluoresces. It is easily excited at 488 nm, and emits at 680 nm; however, it is also well excited at 633 nm (HeNe laser), and thus using Cy5PE reagents in two laser-system requires interlaser compensation.
Many applications and instruments were developed specifically for PE-Cy5. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, PE-Cy5 is also well suited for immunohistochemistry (IHC) and Western blot applications.
Specifications
Product Name
LumiDye™ PE-Cy5
Sinónimos
PE conjugated Cy5 | Phycoerhthrin-Cy5 Conjugate | PE-Cy5 Tandem | Cy5-PE | PE-Cyanine 5.0 | Phycoerythrin-Cyanine 5.0
Grado
Em:670nm, Ex:565nm
Especificaciones y pureza
Ex:565nm, Em:670nm, See COA
Ex/Em(nm)
Peso molecular previsto
240 kDa
Tipo de molécula
Antibody conjugate
Almacenamiento y envío
Concentración
See COA
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
Product should be stored at 2–8℃ in the dark. DO NOT FREEZE.
Imágenes
PE-Cy5 (rp185192) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (PE-Cy5) (rp188522) at 0.05μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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