GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mitochondrial respiratory chain complex V, also known as F₁F₀-ATP synthase, consists of two subunits, F₁ and F₀. It is a key enzyme for ATP synthesis in mitochondrial oxidative phosphorylation and chloroplast photophosphorylation, widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. This enzyme utilizes the proton electrochemical gradient generated by the respiratory chain to catalyze ATP synthesis and can also hydrolyze ATP through a reverse reaction.
The detection principle of this kit is based on the hydrolysis of ATP by mitochondrial respiratory chain complex V, producing ADP and inorganic phosphate (Pi). In the presence of a reducing agent, Pi reacts with ammonium molybdate to form a dark blue phosphomolybdenum complex. By measuring the change in absorbance at 660 nm, the activity of mitochondrial respiratory chain complex V in the sample can be calculated.
Specifications
Especificaciones y pureza
BioReagent
Grado
BioReagent
Aplicación
Enzyme activity assay
Tipo de muestra
Animal tissues, Plant tissues
Método de detección
Colorimetric Method
Detection instrument
Microplate reader
Detection wavelength
660nm
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ for 6 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
