GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.1.0 mg/mL
ExactAb™, Validated, High performance, 1.0 mg/mL ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Myc tag Antibody, Monoclonal (7D9-4C6-6F10), could be used for WB, ICC, IF, IP and so on. Application: WB: 1/1000 - 1/10000 IF/ICC: 1/100 - 1/500 IP: 1/200 Relevance Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein鈥檚 biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Specifications
Product Name
Myc tag Mouse mAb - Primary antibody, specific to Myc, Mouse IgG1
Sinónimos
c-myc tag antibody | Myc Epitope Tag antibody | avian myelocytomatosis viral oncogene homolog | BHLHE39 | bHLHe39MRTL | Class E basic helix-loop-helix protein 39 | cMyc | c-Myc | myc proto-oncogene protein | Myc | Myc2 | MYCC | myc-related translation/loc
Especificaciones y pureza
ExactAb™, Validated, High performance, 1.0 mg/mL
Especies hospedadoras
Mouse
Especificidad
Myc
Inmunógeno
A synthetic peptide derived from Myc-Tag (EQKLISEEDL).
Control positivo
WB: 11654-myc lysates. ICC/IF: 293 cells transfected with a Myc tag protein. IP: Transfected 293 cell lysates with c-Myc tag fusion protein
Conjugación
Unconjugated
Grado
ExactAb™, High Performance, Validated
Isotipo
Mouse IgG1
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
1.0 mg/mL
Nota
This product is about to be sold out and removed from the shelves. The replacement product is Ab155834. Ab169237
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Myc tag Mouse mAb (Ab116621) - Western Blot All lanes: Myc tag Mouse mAb (Ab116621) at 1/5000 dilution Samples: Lysates at 5 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 29 kDa Observed band size: 32 kDa Exposure time: 0.1 s
Myc tag Mouse mAb (Ab116621) - IP Immunoprecipitation analysis of c-Myc Tag in 1: Untransfected HEK293 cell lysate 2: Transfected HEK293 cell lysate with c-Myc tag fusion protein 3: IP (transfected HEK293 + normal mouse IgG+Protein G agarose) 4: IP (transfected HEK293 + Myc tag Mouse mAb (Ab116621) + Protein G agarose)
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/10000
IP
1/200
IF/ICC
1/100-1/500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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