Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Immunoglobulins are the central effector molecules of adaptive humoral immunity. They are produced by B cells and their terminally differentiated plasma cells and contribute to pathogen clearance, maintenance of immune homeostasis, and the development of immunopathology through a dual ...
Antibodies are structurally complex, highly heterogeneous macromolecules that are sensitive to external conditions and inherently less stable.
ChIP is a powerful tool that uses isolated chromatin and antibodies against the target antigen to determine whether the target binds to a specific DNA sequence or to map its distribution across the genome (ChIP-seq).
Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.
In immunology experiments, it is often necessary to select different secondary antibodies to meet the experimental requirements. Usually, there may be several types of secondary antibodies available for a particular experiment, and the following aspects need to be considered to select the most ...
There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.
There are a variety of antibodies available for detecting target proteins for any purpose. In order to narrow down the choice of antibodies and select the appropriate one, the following factors need to be considered.
What concentration of BSA is recommended for WB experiments?
How many slices can I do with 20μI when I have IHC lab-applicable antibodies?
Do I need to adjust the pH again after diluting EDTA Antigen Repair Solution?
Lysate lysate not lysed, add ripa and shake, then lysed on ice for 20 minutes and then centrifuged and got a gooey mess?
Can the lysate be used without sodium aluminate?
Can the antibody be used in applications that are not described in the package insert?
IHC experiment, wondering what are the antigen repair conditions? Should I use EDTA or potassium citrate?
How should I choose the secondary antibody?
What are the temperature requirements for eluting antibodies in an antibody removal solution? Can the same membrane be eluted more than once?
If the species of my experimental sample is not included in the Reactivity of the antibody, is there any after-sales service available?
How long can I keep it at -20°C? Can it be stored for a short time at 4°C or room temperature? Can it be stored at -80°C?
Can I still use it if I accidentally store it at room temperature for a day?
What does IHC mean on the data sheet? Is it frozen section? Is it different from IHC-F?
Immunocytochemistry (ICC) protocol
Whole mount staining is the staining of small pieces of tissue-usually embryos-without sectioning.
Native ChIP (N-ChIP) Protocol
An isotype control antibody appears like a typical antibody but it does not attach itself to a specific target protein like the primary antibody used in the experiment.
Will Aladdin antibodies recognize target protein in complex biological fluids such as blood or serum?
Will Aladdin antibodies recognize target proteins sold by other vendors?
Will Aladdin antibodies work in immunohistochemistry and immunocytochemistry applications?
Do Aladdin’s antibody products contain any carrier proteins or other additives?
What information should be known about the stability of your antibody products?
Are Aladdin’s antibodies suitable for use in ELISA and Western Blot applications?
Have Aladdin’s antibodies been tested in neutralization assays?
Can you tell me what epitope your antibody binds to?
How are your antibodies purified?
Which isotype are Aladdin’s polyclonal antibodies?
IgG, the key factor of Humoral immunity response, is the main immunoglobulin in blood, lymph, cerebrospinal fluid and peritoneal fluid. IgG accounts for approximately 75% of total serum immunoglobulins and is the only immunoglobulin that can penetrate the human placenta. It is primarily ...
Techniques often explored alongside immunological experiments.
