GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), See COA
Mecanismos bioquímicos y fisiológicos
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Especies hospedadoras
Mouse
Especificidad
Human CD3
Inmunógeno
Recombinant Human CD3 Proteins
Control positivo
Flow Cytometry: Human peripheral blood lymphocytes.
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant CD3 Antibody (Ab220319) - Flow Cytometry Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes with Recombinant CD3 Antibody (Ab220319) at 1 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD3 Antibody (Ab220319) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD3 with Recombinant CD3 Antibody (Ab220319) (right panel) at 1 μg/mL for 1 hour at 4 °C, compared with Mouse IgG Isotype Control Antibody (Ab170221) (left panel) and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488).
Aplicación
Aplicación
Dilution info
Flow Cytometry
≤0.5 µg per million cells in 100 µl volume
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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