Recombinant Human 5'-nucleotidase/CD73 Protein, ≥95% (SDS-PAGE)

Cat. No.: rp144096
Disponible para pedir
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. His Tag ? His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. ≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P21589-1
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Immobilized Human CD73 at 2 μg/mL (100 μL/well) can bind CD73 antibody with a linear range of 0.2-2.8 ng/mL.
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
rp144096-10μg
3
139,90US$
50μg
rp144096-50μg
3
539,90US$
100μg
rp144096-100μg
2
869,90US$
1mg
rp144096-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
4.949,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Animal Free,Carrier Free,Bioactive,ActiBioPure™,Azide Free,His-Tag,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Azide Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Specifications

Product Name
Recombinant Human 5'-nucleotidase/CD73 Protein, ≥95% (SDS-PAGE)
Sinónimos
5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase, ecto; 5' nucleotidase, ecto (CD73); 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5
Grado
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, His Tag
Especificaciones y pureza
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥95%(SDS-PAGE)
Pureza
≥95% (SDS-PAGE)
Bioactividad
Immobilized Human CD73 at 2 μg/mL (100 μL/well) can bind CD73 antibody with a linear range of 0.2-2.8 ng/mL.
Concentración de endotoxinas
<0.1 EU/μg
Sistema de expresión
HEK293
Especie
Human
Aminoácidos
1-547 aa
Secuencia
MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKHHHHHH
Etiqueta proteínica
C-His
Número de adhesión
Peso molecular previsto
61 kDa
SDS-PAGE
60-70 kDa, under reducing conditions
Tipo de molécula
Protein
Almacenamiento y envío
Forma
Lyophilized
Reconstitución
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Imágenes

Recombinant Human 5'-nucleotidase/CD73 Protein (rp144096) - SDS-PAGE
2 μg/lane of Recombinant Human 5'-nucleotidase/CD73 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 60 kDa.

Recombinant Human 5'-nucleotidase/CD73 Protein (rp144096) - Protein Bioactivity
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp144096) at 2 μg/mL can bind CD73 antibody, the ED₅₀ for this effect is 2.0 ng/mL.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ25F0724644Certificate of AnalysisJun 22, 2026 rp144096
ZJ25F0724645Certificate of AnalysisJun 22, 2026 rp144096
ZJ25F0724646Certificate of AnalysisJun 22, 2026 rp144096
Preguntas frecuentes y artículos
Calculadoras de soluciones
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