GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Accession #
P21589-1
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only
Especificaciones y pureza
Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥95%(SDS-PAGE), See COA
Mecanismos bioquímicos y fisiológicos
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. C
Bioactividad
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
61.1 kDa, under reducing conditions; 61.7 kDa, under non-reducing conditions.
Tipo de molécula
Protein
Almacenamiento y envío
Concentración
See COA
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
Imágenes
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC₅₀ of 13.1 ng/mL.
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC₅₀ of 2.1 ng/mL.
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - SDS-PAGE 1 μg/lane of Recombinant Human 5'-nucleotidase/CD73 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 61.1 kDa under reducing conditions and 61.7 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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