GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P08887
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC50 of 43.35 ng/mL. 2. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC50 of 67.99 ng/mL. 3. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC50 of 21.05 ng/mL.
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Pureza: ≥95%, por SDS-PAGE visualizado con tinción azul Coomassie®. Función de la proteína: El factor multifuncional interleucina 6 (IL-6) ejerce sus actividades a través de la unión a un complejo receptor de alta afinidad formado por dos glicoproteínas de membrana: un receptor componente de 80 kDa que se une a la IL-6 con baja afinidad (IL-6R alfa ) y un componente de 130 kDa que transduce señales (gp130) que no se une a la IL-6 por sí mismo, pero que es necesario para la unión de alta afinidad de la IL-6 por el complejo. Ambos componentes del complejo receptor, IL-6R alfa y gp130, han sido clonados, secuenciados y expresados. Se ha encontrado una forma soluble del IL-6R alfa en la orina de humanos adultos sanos. Este receptor soluble parece proceder de la escisión proteolítica del IL-6R alfa unido a la membrana. No se ha descrito ningún ARNm natural que codifique una forma truncada del IL-6R alfa. Sin embargo, se han construido formas solubles de IL-6R alfa humanos y murinos mediante la inserción de codones de terminación en las regiones de los ADNc de IL-6R alfa que codifican las porciones externas de los receptores y antes de los dominios transmembrana. Estos receptores solubles se han expresado en células COS-7 y CHO y se ha demostrado que se unen a la IL-6 en solución y que aumentan la actividad de la IL-6 como resultado de la unión del complejo IL-6/IL-6R alfa a la gp130 unida a la membrana
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only
Especificaciones y pureza
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE)
Bioactividad
1. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC50 of 43.35 ng/mL. 2. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC50 of 67.99 ng/mL. 3. Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC50 of 21.05 ng/mL.
60.0-80.8 kDa, under reducing conditions; 60.0-80.7kDa, under non-reducing conditions
Tipo de molécula
Protein
Almacenamiento y envío
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a -20°C durante 1 año. Evitar el ciclo de congelación/descongelación.
Imágenes
Recombinant Human IL-6R alpha Protein (rp181252) - Binding Activity Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 2.0 μg/mL can bind Recombinant Human IL-6 Protein (rp156005) with the EC₅₀ of 43.35 ng/mL.
Recombinant Human IL-6R alpha Protein (rp181252) - ELISA Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Vobarilizumab (anti-IL-6Ra) (Ab175700) with the EC₅₀ of 67.99 ng/mL.
Recombinant Human IL-6R alpha Protein (rp181252) - ELISA Immobilized Recombinant Human IL-6R alpha Protein (rp181252) at 1.0 μg/mL can bind Tocilizumab (anti-IL-6R) (T412004) with the EC₅₀ of 21.05 ng/mL.
Recombinant Human IL-6R alpha Protein (rp181252) - SDS-PAGE 2 μg/lane of Recombinant Human IL-6R alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 60.0-80.8 kDa under reducing conditions and 60.0-80.7 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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