GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P80188
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant Human Lipocalin-2/NGAL Protein (rp176712). The BC50 for this effect is 2.2 μM.
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Función de la proteína: Proteína transportadora de hierro implicada en múltiples procesos como la apoptosis, la inmunidad innata y el desarrollo renal. Se une al hierro mediante la asociación con el ácido 2,5-dihidroxibenzoico (2,5-DHBA), un sideróforo que comparte similitudes estructurales con la enterobactina bacteriana, y libera o elimina hierro de la célula, dependiendo del contexto. La forma unida al hierro (holo-24p3) se internaliza tras la unión al receptor SLC22A17 (24p3R), lo que provoca la liberación de hierro y el consiguiente aumento de la concentración intracelular de hierro. Por el contrario, la asociación de la forma libre de hierro (apo-24p3) con el receptor SLC22A17 (24p3R) va seguida de la asociación con un sideróforo intracelular, la quelación del hierro y la transferencia de hierro al medio extracelular, reduciendo así la concentración intracelular de hierro. Interviene en la apoptosis debida a la privación de interleucina 3 (IL3): la forma cargada de hierro aumenta la concentración de hierro intracelular sin promover la apoptosis, mientras que la forma sin hierro disminuye los niveles de hierro intracelular, induciendo la expresión de la proteína proapoptótica BCL2L11/BIM, lo que provoca la apoptosis. Interviene en la inmunidad innata, posiblemente secuestrando hierro, lo que conduce a limitar el crecimiento bacteriano.
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only
Especificaciones y pureza
ActiBioPure™, bioactivo, sin animales, sin portador, ≥95%(SDS-PAGE)
Bioactividad
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant Human Lipocalin-2/NGAL Protein (rp176712). The BC50 for this effect is 2.2 μM.
25.6 kDa, under reducing conditions; 25.6 kDa, under non-reducing conditions
Tipo de molécula
Protein
Almacenamiento y envío
Reconstitución
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a -20°C durante 1 año. Evitar el ciclo de congelación/descongelación.
Imágenes
Recombinant Human Lipocalin-2/NGAL Protein - Protein Bioactivity Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3] that is prepared using Iron III (Fe3+) (I141291) and DHBA (D154867). The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant Human Lipocalin-2/NGAL Protein (rp176712). The BC50 for this effect is 2.2 μM.
Recombinant Human Lipocalin-2/NGAL Protein (rp176712) - SDS-PAGE 1 μg/lane of Recombinant Human Lipocalin-2/NGAL Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 25.6 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0708526
