GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, Recombinant, ExactAb™, KD Validation, Validated, High Performance, See COA
Mecanismos bioquímicos y fisiológicos
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitinat
Especies hospedadoras
Rabbit
Especificidad
NEDD4L
Inmunógeno
A synthesized peptide derived from human NEDD4-2 (AA 480-602).
Carrier Free, ExactAb™, High Performance, KD Validation, Recombinant, Validated
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant NEDD4L Antibody (Ab327032) - Western Blot
All lanes: Recombinant NEDD4L Antibody (Ab327032) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 119 kDa
Exposure time: 47.2 s
Recombinant NEDD4L Antibody (Ab327032) - Western Blot
Western blotting analysis using Recombinant NEDD4L Antibody (Ab327032). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Recombinant NEDD4L Antibody (Ab327032) (1/20000) and Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) (1/20000) respectively.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ26F0636005
