ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti V5 tag Antibody, Recombinant, could be used for WB, IF/ICC, Flow Cytometry, IP and so on.
Recombinant V5 tag Antibody (Ab133828) - Western Blot
All lanes: Recombinant V5 tag Antibody (Ab133828) at 1/5000 dilution
Samples: Recombinant V5 protein at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
Exposure time: 0.1 s
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - IF/ICC
IF analysis of V5 tag (green) in 293 cells. The cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant V5 tag Antibody (Ab133828) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant V5 tag Antibody (Ab133828) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Red) and C-terminal (Green) Recombinant V5 tag Antibody (Ab133828). The cells were then incubated in Recombinant V5 tag Antibody (Ab133828) (1/10000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant V5 tag Antibody (Ab133828) - Western Blot
All lanes: Recombinant V5 tag Antibody (Ab133828) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 52, 116 kDa
Observed band size: 52, 116 kDa
Exposure time: 4.3 s
| Aplicación | Dilution info |
|---|---|
| IP |
1/50
|
| IF/ICC | 1/2000 - 1/10000 |
| Flow Cytometry | 1/2000 - 1/10000 |
| WB | 1/1000-1/3000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ24F0303636 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
| ZJ24F0303635 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
| ZJ24F0303634 | Certificate of Analysis | Mar 22, 2024 | Ab133828 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide → View High Performance grade guide →
