25µmol/L for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Ex(nm):568 Em(nm):591
Product description
ROX Reference Dye is generally used on Real Time PCR amplification machines of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. Different instruments require different concentrations of ROX Reference Dye. Our company provides ROX Reference Dye storage solution with a concentration of 25μmol/L, and the final concentration of use is 50nmol/L or 500nmol/L depending on the instrument. The user can add it according to the recommended concentration of the instrument.
The recommended final concentration of ROX for different instruments:
Instrument | ROX per 50µL PCR Reaction | Final Concentration |
ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus | 1.0 µL（0.6~1.0µL） | 500nmol/L（300~500nmol/L） |
ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett Rotor Gene 3000 | 0.1 µL*（0.06~0.1µL） | 50nmol/L（30~50nmol/L） |
Subtract the volume of ROX dye from the aqueous solution used to prepare the PCR reaction
*In order to accurately add 0.1μL to each reaction, it is recommended to dilute ROX 10 times with water before use, and use 1μL of the diluted solution.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 I2314345 | Certificate of Analysis | Feb 05, 2026 | R272949 |
| F2417213 | Certificate of Analysis | Jan 20, 2026 | R272949 |
| E2428394 | Certificate of Analysis | Mar 27, 2024 | R272949 |
| G2303293 | Certificate of Analysis | Jun 15, 2023 | R272949 |
| Sensibilidad | Sensitive to light and humidity |
|---|
