Sudan Black B Staining Solution - BioReagent,for microscopy,Biological Stain , CAS No.4197-25-5

CAS: 4197-25-5 Cat. No.: S1508362 Peso molecular: 456.54 Beilstein Registry Number: 723248 Número EC: 224-087-1 PubChem CID: 61336

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Descripción general

There are various methods for neutral fat staining in the laboratory, such as Sudan Black B, Sudan Ⅱ, Sudan Ⅲ, Sudan Ⅳ, and Oil Red O staining. Sudan dyes are generally used in traditional methods, while the azo dye Oil Red O is now more suitable for fat staining. Oil Red O is a highly effective lipid solvent and lipochrome; although its binding affinity to phospholipids is relatively weak, it readily binds to triglycerides to form small lipid droplets. Sudan Black B Stain is mainly used to demonstrate abnormal deposition of lipids, fatty degeneration of tissues and organs, as well as the fatty degeneration commonly occurring in parenchymal organs such as the liver, kidneys, and heart, where numerous neutral fat droplets appear within cells. It also aids in the identification and diagnosis of tumors arising in adipose tissue and their pathological properties. During the experiment, fixatives containing ethanol should not be used for specimens. If fixation is necessary, 10% formalin can be applied. In addition, paraffin sections are not recommended; instead, carbowax sections or frozen sections should be adopted.

S1508362	Component	2×50 mL	2×100 mL	Storage
S1508362A	Sudan Black B Stain	50 mL	100 mL	RT. Store in the dark
S1508362B	Nuclear Fast Red Stain	50 mL	100 mL	RT. Store in the dark

Operating Procedures (For Reference Only)

1. repare cryosections of fresh tissues at a temperature typically ranging from -20℃ to -25℃. For lipoma specimens, adjust the temperature to -30℃.

2. Cut frozen sections with a thickness of 5–10 μm (6–8 μm is optimal) and mount them on glass slides.

3. Rinse the sections briefly with 70% ethanol.

4. Immerse the sections in Sudan Black B Stain for staining.

5. Wash away excess stain with 70% ethanol.

6. Rinse the sections with distilled water for 1 minute.

7. Stain the cell nuclei lightly with Nuclear Fast Red Stain.

8. (Optional) If overstaining is observed under the microscope, differentiate the sections with acid differentiation solution for a few seconds.

9. Rinse briefly with tap water, followed by a quick rinse with distilled water.

10. Blot excess water from the sections and their surroundings with filter paper and allow them to air-dry slightly.

11. Mount the sections with glycerin jelly or gum arabic.

Staining Results

Neutral Fat: Black

Phospholipids Grayish: Black

Cell Nuclei: Red

Precautions

1. Do not use ethanol-containing fixatives or prepare paraffin sections for specimens; frozen sections are required.

2. To prevent dye precipitation during staining, seal the container when immersing sections in the stain. Avoid contact with flowing air to prevent precipitation caused by solvent evaporation.

3. Frozen sections stain easily. Avoid overstaining when counterstaining with Mayer's hematoxylin.

4. Sudan dyes are prone to fading and should be stored in a sealed environment.

5. Specimens mounted with glycerin jelly have a short shelf life. For long-term preservation, seal the edges between the coverslip and slide with neutral balsam.

Specifications

Especificaciones y pureza

BioReagent, for microscopy, Biological Stain

Estabilidad y almacenamiento

Each component has a shelf life of 1 year under corresponding storage conditions.

Condiciones de almacenamiento de almacenamiento

Protected from light, Room temperature

Enviado en

Normal

Grado

Biological Stain, BioReagent, for Microscopy

Nombres e identificadores

• Isómeros SMILES: CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N=NC6=CC=CC=C6)C=CC=C3N1)C
• WGK Alemania: 3
• RTECS: SD4431500
• PubChem CID: 61336
• Peso molecular: 456.54
• Beilstein: 723248
• Reaxy-Rn: 8878215

Propiedades químicas y físicas

• Sensibilidad: Light-sensitive
• Punto de fusión (°C): 120-124°C

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