Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
TSA, or Tyramide Signal Amplification, is a signal amplification technique based on HRP (Horseradish Peroxidase)-catalyzed reactions. It is commonly used in IHC (Immunohistochemistry), ICC (Immunocytochemistry), ISH (In Situ Hybridization), FISH (Fluorescence In Situ Hybridization), and ...
Tyramide Signal Amplification (TSA) is one of the most representative enhancement strategies currently used in in situ fluorescence detection. Its core value is not simply to increase fluorescence intensity, but to convert local signals around low-abundance targets into a high-density, spatially ...
Cyanine 3 Tyramide (Cy3 Tyramide): Principles, Applications, and Experimental Selection Guide for Tyramide Signal Amplification (TSA)
Multiplexed immunohistochemistry (mIHC) is an in situ multiple labeling method based on tyramide signal amplification (TSA) technology, which can achieve simultaneous detection of multiple targets (usually 6-8 types) on a single tissue section. Its core principle is to covalently bind the target ...
Techniques often explored alongside immunological experiments.
