Tissue Washing Solution - BioReagent,sterile,for cell culture

Cat. No.: O1521152

CALIDAD & PUREZA BioReagent, for Cell culture, Sterile

Synonyms

Tissue Wash Buffer

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Descripción general

This product is used for washing tumor or normal tissues during tissue pretreatment. It contains a combination of multiple antibiotics to exert synergistic antibacterial effects, which can effectively reduce contamination rates in primary cell culture.

Instructions for Use

1. Take the digestive enzyme out of the -20℃ refrigerator in advance and thaw it at 2–8℃.

2. Dilute Tissue Digestion Enzyme (Catalog No. O1521148) at a ratio of 1:1 with Digestive Enzyme Diluent (Catalog No. D1521149) to prepare working solution.

3. Transfer tissues into a petri dish, add 3 mL tissue washing solution to rinse tissues, and repeat rinsing 2–3 times.

4. Under aseptic conditions, mechanically cut tissues into 1–2 mm³ fragments, then transfer them into sterile 15 mL centrifuge tubes.

5. Add 5 mL tissue washing solution and centrifuge at 1500 rpm at room temperature for 3 minutes.

6. Discard the supernatant, add 12 mL working-concentration digestive enzyme. Label sample names and numbers, seal the tubes, and place them in a constant-temperature shaking incubator for digestion. Set parameters at 37℃, 200–300 rpm (adjust speed according to tissue texture). Terminate digestion when tissues become flocculent with no visible hard lumps.

7. Take out digested samples and centrifuge at 1500 rpm at room temperature for 3 minutes.

8. Discard the supernatant, add 6 mL serum-containing medium to terminate digestion, and centrifuge at 1500 rpm at room temperature for 3 minutes.

9. Discard the supernatant, resuspend cells with primary cell medium for subsequent use.

Precautions

1. Strict aseptic operation is required during use to avoid contamination.

2. Use the product within the shelf life. Ideal experimental results cannot be guaranteed after expiration.

3. For research use only, not for in vitro diagnosis.

Especificaciones

Sinónimos

Tissue Wash Buffer

Especificaciones y pureza

BioReagent,sterile,for cell culture

Estabilidad y almacenamiento

Store at -20℃ long term (12 months).

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, for Cell culture, Sterile

Product Line

Productos biológicos

Application Protocols

Below are example, general-purpose wash protocols for tissues and cells. Adjust to your application and consult the item’s CoA/Spec Sheet for composition-dependent steps. Sterility and research-use-only restrictions apply.

• Cell culture wash (adherent cells; general)
  1. Aspirate spent medium. 2) Add sufficient Tissue Washing Solution to fully cover the monolayer (e.g., 1–2 mL per well of a 6-well plate). 3) Gently rock 30–60 s. 4) Aspirate. 5) Repeat 1–2× as needed. Proceed to dissociation, staining, or fresh medium.
• Cell pellet wash (suspension or detached cells)
  1. Add 5–10× pellet volume of solution. 2) Gently invert to resuspend. 3) Centrifuge at 200–400 × g for 3–5 min (as appropriate for cell type). 4) Decant supernatant. 5) Repeat wash if necessary.
• Tissue rinse (general)
  1. Place dissected tissue in a chilled tube with ample solution. 2) Gently invert or pipette to dislodge debris. 3) Replace with fresh solution 1–3×. Keep on ice if reduced enzymatic activity is desired.
• Notes and cautions
  • Temperature choice (cold vs ambient) depends on protocol goals.
  • If downstream steps require/avoid divalent cations, verify composition (not specified for this item).
  • Maintain aseptic technique to preserve sterility. Dispose of biohazardous waste per facility SOPs.

Biological Roles

While not a biomolecule, a tissue washing solution plays an enabling role in biological workflows by providing an isotonic, buffered environment that protects tissues and cells during handling.

• Functional roles in the lab (general)
  • Maintains osmotic balance to reduce cell lysis or swelling during washes and transfers.
  • Stabilizes pH near physiological values to preserve protein conformation and cellular integrity during short handling steps.
  • Facilitates removal of interfering substances (serum proteins, enzymes, fixatives, stains) that could impact assays (e.g., immunolabeling, enzymatic measurements, flow cytometry).
  • Supports gentle mechanical trituration, filtration, and sedimentation steps during sample preparation.
• Composition note
  • Common tissue-wash buffers include PBS- or HBSS-like formulations with defined ionic strength; however, the exact composition for this item is not specified. Users should consult the CoA/Spec Sheet when cation content or buffering species are critical to downstream biology (e.g., calcium-dependent adhesion, chelator-sensitive enzymes).
• Application implications
  • Temperature selection (cold vs ambient) can modulate metabolic rates and protease activity during processing; choose per protocol.
  • Pre-equilibration (e.g., to CO2 atmosphere when bicarbonate-buffered) may be relevant for certain buffers; applicability is unknown for this item.

Buffer Applications

This product functions as a wash buffer in tissue and cell workflows. Exact formulation (salts, buffering system, divalent cations) is not specified; verify via CoA/Spec Sheet when application-critical.

• Typical use cases (general guidance)
  • Washing cells/tissues between enzymatic dissociation, staining, or fixation steps to remove residual reagents.
  • Preconditioning cultureware and scaffolds to isotonic conditions prior to seeding.
  • Rinsing biopsy or dissection samples to reduce blood/carryover before downstream processing.
• Practical parameters (general; not item specifications)
  • Temperature: Use 2–8°C to slow metabolism/proteolysis during dissections; room temperature to avoid cold shock for sensitive cells.
  • Volumes and cycles: 2–3 washes with 5–10× sample volume are commonly effective. Gentle inversion or low-speed centrifugation (e.g., 200–400 × g for adherent-derived suspensions) can aid reagent removal.
  • Divalent cations: Presence/absence of Ca2+/Mg2+ affects cell adhesion and enzyme activity; confirm for this item before critical applications.
  • Filtration: If sterility is in doubt after handling, re-filter through a 0.22 µm sterile filter in a biosafety cabinet (if compatible with this item’s packaging and your sterility SOPs).
• Caveats
  • pH/osmolality targets for your assay should be confirmed experimentally since they are not specified for this product.
  • For electrophoresis or specialized buffer systems, choose a defined formulation with known ionic strength and buffering capacity.

Green Alternatives

This is an aqueous, water-based laboratory solution; relative to organic solvents, it is inherently aligned with green chemistry principles for the intended application.

• Sustainability perspective (general)
  • Water-based, non-flammable medium minimizes VOC emissions and fire risk compared with organic washing solvents.
  • Use right-sized packaging and aliquots to reduce waste from thawed, unused material.
  • Cold-chain shipping is necessary to preserve quality; consolidate orders where feasible to reduce environmental footprint.
• Comparison (general; not item-specific)
  • Versus organic rinses (e.g., methanol, acetone): Aqueous washes are gentler on tissues/cells and generate less hazardous waste, but they may be less effective for removing strongly hydrophobic contaminants.
  • Versus dry-ice shipping: Ice-pad shipment as provided can reduce CO2 emissions; always return promptly to recommended storage to avoid waste.
• Practical tips
  • Plan workflows to minimize repeated freeze–thaw cycles (aliquot once, use-once) to extend product life and reduce disposals.
  • Confirm local waste management guidance for saline/aqueous buffers that contacted biohazardous materials.

Pharmaceutical Uses

This product is labeled For research use only and is not intended for human or veterinary use, diagnostics, or clinical applications.

• General industry context (literature/general)
  • Similar buffered saline solutions are widely used as process buffers in non-clinical biomanufacturing (equipment rinses, intermediate washes) and in analytical sample preparation.
  • Key attributes often sought for GMP/process buffers—such as defined composition, bioburden/endotoxin limits, and validation of sterilization—are application-specific and require documented specifications.
• Item-specific clarifications
  • Pharmacopeial status, excipient listing, GMP compliance: Not specified for this item; refer to CoA/Spec Sheet.
  • This SKU (O1521152) is not represented as a clinical-grade material.
• Practical guidance for research labs
  • If transitional use in process development is anticipated, verify lot-to-lot consistency and request detailed specifications/CoA.
  • For any regulated or clinical manufacturing setting, source a buffer with explicit GMP documentation and pharmacopeial compliance appropriate to the application.

Physical Properties

Because this is a formulated aqueous mixture, single-compound physical constants are not applicable.

• Item-specific properties
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • pH, osmolality, conductivity: Not specified for this item; refer to CoA/Spec Sheet.
  • Density, refractive index: Not specified for this item; refer to CoA/Spec Sheet.
  • Solubility: Aqueous solution (ready-to-use). Miscible with water; compatibility with organic solvents is application-dependent (general guidance).
• Literature/general expectations for tissue-wash buffers (not item specifications)
  • Physical state: Clear, colorless aqueous solution (typical).
  • Typical osmolality: ~280–320 mOsm/kg (literature for isotonic buffers).
  • Typical pH ranges: ~7.2–7.6 for common physiological buffers (literature); exact pH for this item is not specified.
  • Freezing/thawing: Aqueous saline buffers may freeze at or below 0°C; repeated freeze–thaw can increase risk of container stress and contamination; follow storage guidance below.
• Important note
  • Do not treat the above literature values as specifications. For exact composition and specifications of SKU O1521152, consult the product’s CoA/Spec Sheet and SDS.

Quality and Grades

• Item-specific quality statements (from Product Data)
  • Grade/Purity: BioReagent, for Cell culture, Sterile
  • Research Use: For research use only
• What these designations generally imply (literature/general guidance)
  • BioReagent grade: Suitable for biological research workflows, manufactured with attention to common biochemical contaminants; however, specific impurity limits for this item are not provided.
  • For Cell culture: Formulated and processed to be compatible with in vitro cell handling. Typical industry expectations may include low bioburden and sterility; specific parameters (e.g., endotoxin, mycoplasma) are not specified for this item.
  • Sterile: Provided sterile as packaged. Maintain sterility by using aseptic technique and sterile tools. Do not assume sterility after opening unless handled aseptically.
• Documentation and specifications
  • Exact composition, pH, osmolality, endotoxin levels, sterility validation method, and permissible bioburden: Not specified for this item; refer to CoA/Spec Sheet.
• Practical guidance
  • On first use, consider aseptic aliquoting to single-use volumes to limit freeze–thaw and handling exposures.
  • If using in sensitive cultures (primary cells, stem cells), confirm compatibility with a small pilot prior to broad adoption.
  • Track lot numbers and retain CoAs for experimental traceability.

Reaction and Applications

This product is not intended for chemical synthesis reactions; it is a process reagent for tissue/cell handling.

• Intended laboratory uses (general; expand on manufacturer’s category “life science”)
  • Tissue/cell washing to remove serum, enzymes (e.g., dissociation reagents), fixatives, dyes, or debris between workflow steps.
  • Sample conditioning prior to lysis, nucleic acid/protein extraction, or flow cytometry—providing an isotonic environment to minimize osmotic shock.
  • Pre-rinse of cultureware or tissues to control background in immunostaining or histology.
• Practical tips (general best practices)
  • Temperature: Use chilled buffer (on ice or 2–8°C) to slow enzymatic activity during tissue dissection, or room temperature when avoiding cold shock—follow your protocol.
  • Volumes: Employ generous wash volumes (e.g., 5–10× sample volume) and gentle mixing to maximize removal of residual reagents without damaging fragile samples.
  • Divalent cations: If enzymatic dissociation or chelation is involved, ensure the washing solution’s cation profile is compatible. Composition for this item is not specified; confirm via CoA or a small-scale pilot.
  • Sterility: Use sterile technique throughout to prevent contamination of downstream cell culture steps.
• Not for therapeutic use: For research use only, as stated in the Product Data.

Reaction Conditions

Not applicable. This product is not intended for use as a reaction medium or reagent in chemical synthesis.

• General guidance
  • If used as a wash in analytical or bioassay workflows, follow method-specific conditions (temperature, contact time, mixing). Since formulation details are not specified for this item, confirm compatibility with sensitive assays on a small scale first.

Safety and Handling

Safety data for this specific formulation are not provided in the Product Data. Always review the product SDS for authoritative information.

• Item-specific hazard info
  • GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• General handling (aqueous, cell-culture–grade sterile buffer)
  • PPE: Wear lab coat, gloves (nitrile/latex), and safety glasses. Use sterile technique to maintain product integrity.
  • Contamination control: Work in a clean area or biosafety cabinet when opening/aliquoting. Avoid touching sterile closures with non-sterile tools.
  • Incompatibilities: Avoid microbial or chemical contamination (e.g., do not return used aliquots to stock). Avoid mixing with reactive chemicals or strong oxidizers unless validated by protocol.
  • First aid (general guidance for neutral aqueous solutions): In case of eye/skin contact, rinse with water. If inhaled as aerosol, move to fresh air. If ingested, rinse mouth with water. Seek medical attention if irritation persists. Defer to SDS for specifics.
  • Spill response: Absorb with inert material, clean with water and laboratory disinfectant if appropriate for your facility.
• Storage and transport
  • Store at −20°C as specified. Protect from light if the container is ambered; minimize freeze–thaw cycles by aliquoting upon first thaw (general best practice).
  • Shipped cold (ice chest + ice pads); inspect upon receipt and return to recommended storage promptly.

Solvent Selection

This product is a ready-to-use aqueous washing solution rather than an organic solvent. Traditional “solvent selection” criteria are not directly applicable.

• General properties (literature/general)
  • Polarity: Highly polar; water-based.
  • Miscibility: Miscible with water and other common aqueous buffers. Mixing with organic solvents (e.g., ethanol, DMSO) may alter osmolar balance and is generally not recommended unless protocol-validated.
• When to choose a tissue washing solution
  • For rinsing tissues or cells during processing, media changes, dissociation workflows, or pre-/post-staining washes where isotonic, near-physiological conditions are required.
• Comparisons (general; not item-specific)
  • Many labs choose between PBS, HBSS, or custom saline-based buffers for washing. Selection depends on the need for divalent cations (Ca2+/Mg2+), bicarbonate/CO2 buffering, and downstream enzyme compatibility. Composition for this item is not specified; verify before use where cation content matters (e.g., cell adhesion or trypsin activity).

Storage and Reconstitution

• Item-specific storage (from Product Data)
  • Store at −20°C. Shipped in ice chest with ice pads. Keep sterile.
• Handling after receipt (general best practices)
  • Upon arrival, promptly transfer to −20°C storage. Avoid prolonged exposure to elevated temperatures.
  • On first thaw, mix gently by inversion to re-homogenize. Do not vortex vigorously if foaming is undesirable.
  • Aliquot under aseptic conditions into sterile, single-use portions to minimize freeze–thaw cycles and contamination risk.
• Stability and shelf life
  • Exact shelf life, allowable freeze–thaw cycles, and in-use stability at 2–8°C or room temperature: Not specified for this item; refer to CoA/Spec Sheet.
  • Protect from microbial contamination after opening; discard if turbidity, precipitate, color change, or container breach is observed.
• Reconstitution
  • The product is a ready-to-use solution; no reconstitution is required. If concentration adjustments or buffer exchanges are needed for your protocol, validate by small-scale testing and sterile filtration (0.22 µm) as appropriate.
• Container/material compatibility (general)
  • Store in original container or sterile polypropylene bottles/tubes. Avoid repeated freeze–thaw of the primary container; use aliquots.
• Documentation
  • For precise storage limits and post-thaw use windows, consult the product’s CoA/Spec Sheet and SDS.

Structure and Identity

This product is a formulated aqueous laboratory reagent supplied as a ready-to-use sterile “Tissue Washing Solution,” not a single defined chemical entity.

• Item-specific facts (from Product Data)
  • SKU: O1521152
  • Product name: Tissue Washing Solution
  • Grade/Purity: BioReagent, for Cell culture, Sterile
  • Storage: Store at −20°C
  • Shipping: Ice chest + ice pads
  • Research use: For research use only
• Mixture/identity considerations
  • Chemical Name: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula and weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / InChIKey: Not applicable for a formulated mixture; not specified for this item; refer to CoA/Spec Sheet.
• Structural description (general knowledge)
  • Typical tissue-wash solutions are aqueous, buffered, and isotonic to support cells/tissues during handling. They commonly contain inorganic salts and buffering components; exact composition for this item is not specified.
  • No discrete 2D molecular structure applies because the product is a multi-component solution (literature/general).

Synthetic Utility

Not typically applicable. This is a formulated aqueous washing solution intended for biological sample handling rather than chemical synthesis.

• General note (literature/general)
  • In synthetic laboratories, aqueous saline buffers may occasionally be used for phase separations, quenching, or washing organic layers; however, composition for this item is not specified, and it is supplied as sterile, cell-culture–grade material. Using dedicated, non-sterile lab-grade salts/buffers is usually more economical for synthetic operations.
• Recommendation
  • For chemical synthesis, select buffers with defined composition and known pH/ionic strength suitable for your reaction or workup.

Target Specificity

Not applicable. Target/epitope specificity, clone, isotype, and species reactivity pertain to antibodies or affinity reagents and are not relevant to a general tissue washing solution.

• Item-specific data
  • No target specificity information is provided or expected for SKU O1521152.