GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Armenian Hamster anti Mouse/Rat TNF alpha Antibody, Monoclonal (TN3-19.12), could be used for Flow, in vivo TNFα neutralization and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo TNFα neutralization: Use at an assay dependent concentration. Protein Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Specifications
Product Name
TNF alpha Armenian Hamster mAb - Primary antibody, specific to TNF alpha, Armenian Hamster IgG
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Especies hospedadoras
Armenian hamster
Especificidad
TNF alpha
Inmunógeno
Recombinant protein of mouse TNFα
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, PBS Only, Validated
Isotipo
Armenian Hamster IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Imágenes
TNF alpha Armenian Hamster mAb (Ab131798) - Flow Cytometry Flow Cytometry analysis of 1.0 μg/mL LPS stimulated RAW 264.7 cells (overnight) labelling TNF alpha (red) with TNF alpha Armenian Hamster mAb (Ab131798) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Armenian Hamster IgG (Biotin) (Ab135991). Black - Unlabelled control, cells without incubation with primary antibody.
TNF alpha Armenian Hamster mAb (Ab131798) - ELISA Immobilized Recombinant Rat TNF-alpha protein (rp183596) at 1.0 μg/mL can bind TNF alpha Armenian Hamster mAb (Ab131798) with the EC50 of 281.9 ng/mL.
Aplicación
Aplicación
Dilution info
Flow Cytometry
Use at an assay dependent concentration.
in vivo TNFα neutralization
Use at an assay dependent concentration
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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