Tricine - for cell culture, ≥99.5%(T), used for Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and

CAS: 5704-04-1 Cat. No.: T118067 Peso molecular: 179.17 Beilstein Registry Number: 1937804 Número EC: 227-193-6 PubChem CID: 79784

GRADE & PURITY for Cell culture, ≥99.5%(T)

Synonyms

2-(1,3-dihydroxy-2-(hydroxymethyl)propan-2-ylamino)acetic acid | C6H13NO5 | 2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid | N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine | AKOS015856413 | 2,6,6-Trimethyl-2-cyclohexen-1-carboxaldehyde

Storage

Room temperature

Shipped In

Normal

Descripción general

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Specifications

Sinónimos

2-(1, 3-dihydroxy-2-(hydroxymethyl)propan-2-ylamino)acetic acid | C6H13NO5 | 2-{[1, 3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}acetic acid | N-[2-hydroxy-1, 1-bis(hydroxymethyl)ethyl]glycine | AKOS015856413 | 2, 6, 6-Trimethyl-2-cyclohexen-1-carboxaldehyde

Especificaciones y pureza

for cell culture, ≥99.5%(T)

Aplicación

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radi

Condiciones de almacenamiento de almacenamiento

Room temperature

Enviado en

Normal

Grado

for Cell culture

Pureza

≥99.5%(T)

Nombres e identificadores

• Pubchem Sid: 504755419
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504755419
• Sonrisas canónicas: C(C(=O)O)NC(CO)(CO)CO
• IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
• InChIKey: SEQKRHFRPICQDD-UHFFFAOYSA-N
• INCHI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Isómeros SMILES: C(C(=O)O)NC(CO)(CO)CO
• WGK Alemania: 3
• PubChem CID: 79784
• Peso molecular: 179.17
• Beilstein: 1937804
• Reaxy-Rn: 1937804

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Clase Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Clase: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Alpha amino acids
• Alternative Parents: Amino acids 1,2-aminoalcohols Monocarboxylic acids and derivatives Dialkylamines Carboxylic acids Primary alcohols Organopnictogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Alpha-amino acid - 1,2-aminoalcohol - Amino acid - Carboxylic acid - Secondary aliphatic amine - Monocarboxylic acid or derivatives - Secondary amine - Organic nitrogen compound - Primary alcohol - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Carbonyl group - Amine - Organic oxygen compound - Alcohol - Aliphatic acyclic compound
• Descripción: This compound belongs to the class of organic compounds known as alpha amino acids. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon).
• External Descriptors: tricine

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Solubilidad: Soluble in water (89.6 g/l) at 20 °C.
• Punto de fusión (°C): 172 °C
• Peso molecular: 179.170 g/mol
• XLogP3: -5.100
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 6
• Exact Mass: 179.079 Da
• Monoisotopic Mass: 179.079 Da
• Topological Polar Surface Area: 110.000 Ų
• Heavy Atom Count: 12
• Formal Charge: 0
• Complexity: 137.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Ziwei Su, Xiangxiang Zhang, Weichen Wang, Mingdong Dong, Xiaojun Han.  (2022)  Light-Harvesting Artificial Cells for the Generation of ATP and NADPH†.  CHINESE JOURNAL OF CHEMISTRY,  41  (1): (57-63).  [PMID:] [10.1002/cjoc.202200482]

2. Ping Sun, Jing Wu, Shenghong Yang, Hongli Li, Lei Zhao, Yuanhang Wang, Xiayan Wang, Qiaosheng Pu.  (2021)  Robust and easy-to-use microchip electrophoresis within sub-millimeter channels for fast and highly efficient separation.  TALANTA,      [PMID:34517615] [10.1016/j.talanta.2021.122747]

3. Yongjia Tang, Haoran Xu, Yaxue Dai, Fang Wang, Wenjing Huang, Peifei Liu, Yueqing Gu.  (2021)  A novel peptide targeting c-Met for hepatocellular carcinoma diagnosis.  Journal of Materials Chemistry B,  9  (22): (4577-4586).  [PMID:34047746] [10.1039/D1TB00408E]

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