Experiments for the determination of growth fraction

Summary

Cells were labeled continuously for 48h and sampled at intervals for radioautography. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition

Operation method

Program 21.12 Experiments for the Determination of Growth Fractions

Principle

Cells were labeled continuously for 48h and sampled at intervals for radioautography.

Move

procedure

Except for step 3, the remaining steps are as in the program 21.10 The incubation times should be 15min, 30min and 1, 2, 4, 8, 24, 48h respectively.

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