ASPM Mouse mAb - 1차 항체, specific to ASPM

Cat. No.: Ab089860

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG2b

Clone Number

K52002_11G4

Conjugation

Unconjugated

개요

Rabbit anti Human ASPM Antibody, Monoclonal (K52002_11G4), could be used for WB, IHC and so on.

Specifications

Product Name

ASPM Mouse mAb - 1차 항체, specific to ASPM

동의어

ASPM | ASP | MCPH5 | Calmbp1 | Asp (abnormal spindle) homolog, microcephaly associated antibody | Asp (abnormal spindle) like, microcephaly associated antibody | ASP antibody | Calmbp 1 antibody | Calmbp1 antibody | Calmodulin binding protein 1 antibody |

사양 및 순도

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

숙주 종

마우스

특이성

ASPM

면역원

Recombinant protein of human ASPM (AA 411-720)

포지티브 제어

WB: HEK293 lysate. IHC: Human adrenal gland tissue.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -80°C,Avoid repeated freezing and thawing

배송

Dry ice packs + Cold packs

안정성 및 스토리지

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: ASPM Mouse mAb (Ab089860)

ASPM Mouse mAb (Ab089860) - Western Blot
15 μg of HEK293 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ASPM Mouse mAb (Ab089860) at 1 μg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse lgG was used as the secondary antibody. ASPM band was visualized using ECL Substrate.
Result: ASPM Mouse mAb (Ab089860) can detect ASPM by Western blotting.
Predicted band size: 410 kDa
Observed band size: 410 kDa

IHC: ASPM Mouse mAb (Ab089860)

ASPM Mouse mAb (Ab089860) - IHC
IHC analysis of adrenal gland tissue by ASPM Mouse mAb (Ab089860).
IHC was performed using sections of the formalin-fixed paraffin-embedded adrenal gland tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ASPM Mouse mAb (Ab089860) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

적용

• 적용: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/400

관련 대상(인간)

ASPM Tbio Abnormal spindle-like microcephaly-associated protein (0 Activities)

Discover Target: ASPM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)