BrdU Mouse mAb - 1차 항체, IgG1

Cat. No.: Ab169026

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

IF/ICC

Host species

마우스

Reactive species

종에 독립적인

Isotype

Mouse IgG1

Clone Number

AB02/5B2

Conjugation

Unconjugated

개요

Application
ICC/IF: 0.5μg/mL
Mouse anti BrdU antibody, clone 5B2, is suitable for detecting incorporated BrdU in a wide variety of cell types.

Specifications

Product Name

BrdU Mouse mAb - 1차 항체, IgG1

동의어

Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

숙주 종

마우스

면역원

Bromodeoxyuridine

포지티브 제어

ICC/IF: HeLa cells

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

아이소타입

IgG1

라이트 체인 유형

lambda

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at +4°C short term (1-2 weeks). Store at -20°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

ICC/IF: BrdU Mouse mAb (Ab169026)

BrdU Mouse mAb (Ab169026) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab169026) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab169026) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

적용

• 적용: Dilution info
• IF/ICC:

  0.5 μg/mL ;