Caveolin-1 Mouse mAb - 1차 항체, specific to Caveolin-1, Mouse IgG1

Cat. No.: Ab192266

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, WB

Host species

마우스

Reactive species

인간, 조언

Isotype

Mouse IgG1

Clone Number

CD02/2C9

Conjugation

Unconjugated

개요

Mouse anti Human Caveolin-1 Antibody, clone CD02/2C9, could be used for WB, Flow cytometry, ELISA and so on.

Specifications

Product Name

Caveolin-1 Mouse mAb - 1차 항체, specific to Caveolin-1, Mouse IgG1

동의어

BSCL3 antibody | CAV antibody | CAV1 antibody | CAV1_HUMAN antibody | caveolae protein, 22 kD antibody | caveolin 1 alpha isoform antibody | caveolin 1 beta isoform antibody | Caveolin 1 caveolae protein 22kDa antibody | Caveolin-1 antibody | Caveolin1 an

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

생화학 및 생리적 메커니즘

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-

숙주 종

마우스

특이성

Caveolin-1

면역원

Recombinant protein corresponding to Human Caveolin-1

포지티브 제어

WB: HUVEC, C6 and NIH/3T3 whole cell lysates Flow: HeLa cells

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150.0 KDa

정화 방법

Protein A purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

이미지

Western Blot: Caveolin-1 Mouse mAb (Ab192266)

Caveolin-1 Mouse mAb (Ab192266) - Western Blot
All lanes: Caveolin-1 Mouse Antibody (Ab192266) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 20 kDa
Observed band size: 19, 21 kDa
Exposure time: 30.0 s 

Flow Cytometry: Caveolin-1 Mouse mAb (Ab192266)

Caveolin-1 Mouse mAb (Ab192266) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Caveolin-1 Mouse mAb (Ab192266) (Red) at 5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).

적용

적용

Dilution info

Flow Cytometry

1/200 - 1/3,000

WB

1/2,000 – 1/100,000; Predicted molecular weight: 20.5 KDa

관련 대상(인간)

CAV1 Tbio Caveolin-1 (0 Activities)

Discover Target: CAV1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Caveolin-1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.