CD3 Mouse mAb (AF488) - 1차 항체, specific to CD3, Mouse IgG1

Cat. No.: Ab189661
주문 가능
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:490nm ? Fluorescence excitation maximum at 490 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:525nm ? Fluorescence emission maximum at 525 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 5 μL/test
Application
Flow Cytometry
Host species
마우스
Reactive species
인간
Isotype
Mouse IgG1
Clone Number
UCHT1
Conjugation
AF488
★
Size
USA
독일 (EU)*
Price
Qty
10T
Ab189661-10T
재고 있음 ≥10
—
US$81.90
25T
Ab189661-25T
8 재고 있음
—
US$163.90
100T
Ab189661-100T
5 재고 있음
—
US$409.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test Em:525nm,Ex:490nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

개요

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

Specifications

Product Name
CD3 Mouse mAb (AF488) - 1차 항체, specific to CD3, Mouse IgG1
동의어
CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a
사양 및 순도
ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test
생화학 및 생리적 메커니즘
The CD3 complex mediates signal transduction.
숙주 종
마우스
특이성
CD3
면역원
Human CD3ε
포지티브 제어
Flow: Human peripheral blood lymphocytes
변화
AF488
여기(nm)
Blue Laser(488nm)
방출(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
등급
Em:525nm, Ex:490nm, ExactAb™, Validated
아이소타입
Mouse IgG1
라이트 체인 유형
kappa
정화 방법
Protein G purified
집중력
5 μL/test
보관 및 배송
보관 조건
Store at 2-8°C,Protected from light,Do not freeze
배송
Wet ice,Do not freeze
안정성 및 스토리지
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
이미지

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245).Black - Unlabelled control, cells without incubation with primary antibody.

적용
적용Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

관련 대상(인간)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
작용 메커니즘
인증서(CoA, COO, BSE/TSE 및 분석 차트)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type날짜항목
ZJ24F0910463Certificate of AnalysisApr 07, 2026 Ab189661
ZJ24F0910464Certificate of AnalysisApr 07, 2026 Ab189661
ZJ24F0910465Certificate of AnalysisApr 07, 2026 Ab189661
자주 묻는 질문과 기사
솔루션 계산기
리뷰

고객 리뷰

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:490nm grade guide → View Em:525nm grade guide →

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Mouse IgG3-kappa (APC), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - 아이소타입 대조 항체, specific to HEL
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Mouse IgG2a-kappa (APC), anti-A29L - 아이소타입 대조 항체, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - 아이소타입 대조 항체, specific to HEL
Mouse IgG (PE-AF647) - 아이소타입 대조 항체

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