CD4 Mouse mAb (FITC) - 1차 항체, specific to CD4, Mouse IgG2b

Cat. No.: Ab180131

등급 & 순도 Validated, Ex:498nm, Em:517nm, 5 μL/test

Application

Flow Cytometry

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG2b

Clone Number

OKT4

Conjugation

FITC

개요

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

사양

Product Name

CD4 Mouse mAb (FITC) - 1차 항체, specific to CD4, Mouse IgG2b

동의어

CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti

사양 및 순도

Validated, Ex:498nm, Em:517nm, 5 μL/test

숙주 종

마우스

특이성

CD4

면역원

Human peripheral T cells.

포지티브 제어

Flow: Human peripheral blood lymphocytes

변화

FITC

여기(nm)

Blue Laser(488nm)

방출(nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

등급

Em:517nm, Ex:498nm, Validated

아이소타입

Mouse IgG2b

정화 방법

Protein G purified

집중력

5 μL/test

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at 2-8°C,Protected from light,Do not freeze

배송

Wet ice,Do not freeze

안정성 및 스토리지

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

이미지

Flow Cytometry: CD4 Mouse mAb (FITC) (Ab180131)

CD4 Mouse mAb (FITC) (Ab180131) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (FITC) (Ab180131) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (FITC) (Ab180307).

Flow Cytometry: CD4 Mouse mAb (FITC) (Ab180131)

CD4 Mouse mAb (FITC) (Ab180131) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (FITC) (Ab180131) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

적용

적용

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

관련 대상(인간)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Ex:498nm indicate?

Ex:498nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (FITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.