CD42b Mouse mAb - 1차 항체, specific to CD42b, IgG1

Cat. No.: Ab094818

등급 & 순도 Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG1

Clone Number

6H6H10

Conjugation

Unconjugated

개요

Mouse monoclonal (6H6H10) to CD42b, could be used for WB, Flow and ELISA.
Application:
WB: 1/500 - 1/2000; Predicted molecular weight: 72 kDa.
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function:
Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.

사양

Product Name

CD42b Mouse mAb - 1차 항체, specific to CD42b, IgG1

동의어

Antigen CD42b alpha antibody | Antigen CD42b-alpha antibody | BDPLT1 antibody | BDPLT3 antibody | BSS antibody | CD 42b antibody | CD42b alpha antibody | CD42b antibody | CD42b antigen antibody | DBPLT3 antibody | Glycocalicin antibody | Glycoprotein Ib (

사양 및 순도

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

숙주 종

마우스

특이성

CD42b

면역원

Purified recombinant fragment of human CD42B (AA: extra 17-183) expressed in E.coli

포지티브 제어

WB: HL-60, MOLT-4 and Ramos celll lysates; CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate; Human CD42b (aa 17-183) recombinant protein. FCM: HL-60 and Jurkat cells.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

1.0 mg/mL

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Storage: 4℃; -20℃ for long term storage. Avoid repeated freeze/thaw cycles.

이미지

Western Blot: CD42b Mouse mAb (Ab094818)

CD42b Mouse mAb (Ab094818) - Western Blot
All lanes: CD42b Mouse mAb (Ab094818) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Flow Cytometry: CD42b Mouse mAb (Ab094818)

CD42b Mouse mAb (Ab094818) - Flow Cytometry
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD42b with CD42b Mouse mAb (Ab094818) at 1/200 dilution (green) compared with a negative control (red).

Flow Cytometry: CD42b Mouse mAb (Ab094818)

CD42b Mouse mAb (Ab094818) - Flow Cytometry
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD42b with CD42b Mouse mAb (Ab094818) at 1/200 dilution (green) compared with a negative control (red).

적용

적용

Dilution info

Flow Cytometry

1/200-1/400

ELISA

1/10000

관련 대상(인간)

GP1BA Tbio Platelet glycoprotein Ib alpha chain (0 Activities)

Discover Target: GP1BA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

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