CD45.2 Mouse mAb - 1차 항체, specific to CD45.2, Mouse IgG2a

Cat. No.: Ab094926

등급 & 순도 Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IHC

Host species

마우스

Reactive species

마우스

Isotype

Mouse IgG2a

Clone Number

104.2

Conjugation

Unconjugated

개요

Mouse anti Mouse CD45.2 Antibody, Monoclonal (104.2), could be used for Flow, in vivo CD45.2 blockade, in vitro CD45.2 blockade, IHC and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo CD45.2 blockade: Use at an assay dependent concentration.
in vitro CD45.2 blockade: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

사양

Product Name

CD45.2 Mouse mAb - 1차 항체, specific to CD45.2, Mouse IgG2a

동의어

loc | Cd45 | Ly-5 | Lyt-4 | B220 | CD_antigen: CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-prot

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

숙주 종

마우스

특이성

CD45.2

면역원

B10.S mouse thymocytes and splenocytes

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

아이소타입

Mouse IgG2a

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

See COA

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at 2-8°C,Do not freeze

배송

Wet ice,Do not freeze

안정성 및 스토리지

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

이미지

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 with CD45.2 Mouse mAb (Ab094926) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 (red) with CD45.2 Mouse mAb (Ab094926) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

적용

적용

Dilution info

IHC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD45.2 blockade

Use at an assay dependent concentration.

in vitro CD45.2 blockade

Use at an assay dependent concentration.

자주 묻는 질문

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD45.2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.