CD45RO Mouse mAb - 1차 항체, specific to CD45RO, Mouse IgG2a

Cat. No.: Ab094946

GRADE & PURITY Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Application

Flow Cytometry

Host species

마우스

Reactive species

원숭이, 인간, 일반 마모셋, 침팬지

Isotype

Mouse IgG2a

Clone Number

UCHL1

Conjugation

Unconjugated

개요

Mouse anti Human CD45RO Antibody, Monoclonal (UCHL1), could be used for Flow and so on.
Application:
Flow: 1μg/100w cells
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

Specifications

Product Name

CD45RO Mouse mAb - 1차 항체, specific to CD45RO, Mouse IgG2a

동의어

B220 | CD45 antigen | CD45R | CD45RO | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor type, c polypeptide | B220 a

사양 및 순도

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

숙주 종

마우스

특이성

CD45RO

면역원

IL-2 dependent T cell line, CA1，IL-2

포지티브 제어

Flow: Human peripheral blood lymphocytes.

변화

Unconjugated

등급

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

아이소타입

Mouse IgG2a

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at 2-8°C

배송

Wet ice

안정성 및 스토리지

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

이미지

Flow Cytometry: CD45RO Mouse mAb (Ab094946)

CD45RO Mouse mAb (Ab094946) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45RO (right) with CD45RO Mouse mAb (Ab094946) at 0.1 μg/mL (right panel) compared with mouse IgG (Ab170221) - Isotype Control (left panel). Goat anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 was used as the secondary antibody.

Flow Cytometry: CD45RO Mouse mAb (Ab094946)

CD45RO Mouse mAb (Ab094946) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45RO (red) with CD45RO Mouse mAb (Ab094946) at 0.1 μg/mL for 1 hour at 4°C. Goat anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

적용

적용

Dilution info

Flow Cytometry

1μg/100w cells

관련 대상(인간)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

작용 메커니즘

자주 묻는 질문

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD45RO Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.