CD8 alpha Rat mAb - 1차 항체, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab095344

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IF/ICC, WB

Host species

조언

Reactive species

마우스

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

Unconjugated

개요

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.
Application
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
in vivo CD8+ T cell depletion: Use at an assay dependent concentration.
Protein Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specifications

Product Name

CD8 alpha Rat mAb - 1차 항체, specific to CD8 alpha, Rat IgG2a

동의어

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

사양 및 순도

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

숙주 종

조언

특이성

CD8 alpha

면역원

Mouse Spleen Cells or Thymocyte Membranes

변화

Unconjugated

등급

Azide Free, Carrier Free, PBS Only, Validated

아이소타입

Rat IgG2a

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

See COA

Product Line

생물 제품

보관 및 배송

모양

Liquid

보관 조건

Store at 2-8°C,Do not freeze

배송

Wet ice,Do not freeze

안정성 및 스토리지

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

이미지

Flow Cytometry: CD8 alpha Rat mAb (Ab095344)

CD8 alpha Rat mAb (Ab095344) - Flow Cytometry
Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095344; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

적용

적용

Dilution info

WB

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD8+ T cell depletion

Use at an assay dependent concentration.

관련 대상(인간)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)