GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
개요
Rat anti Mouse CD8 alpha Antibody, Monoclonal (YTS 169.4), could be used for in vivo CD8+ T cell depletion, WB, Flow and so on. Application: in vivo CD8+ T cell depletion: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Specifications
Product Name
CD8 alpha Rat mAb - 1차 항체, specific to CD8 alpha, Rat IgG2b
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
이미지
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095356; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha with CD8 alpha Rat mAb (Ab095356) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
CD8 alpha Rat mAb (Ab095356) - Flow Cytometry Flow Cytometry analysis of mouse splenocytes cells labelling CD8 alpha (red) with CD8 alpha Rat mAb (Ab095356) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
적용
적용
Dilution info
WB
Use at an assay dependent concentration.
Flow Cytometry
Use at an assay dependent concentration.
in vivo CD8+ T cell depletion
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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