CD99 Mouse mAb - 1차 항체, specific to CD99, Mouse IgG1

Cat. No.: Ab006798

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

Flow Cytometry, IHC, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG1

Clone Number

1B10E12

Conjugation

Unconjugated

개요

Mouse anti Human CD99 Antibody, Monoclonal, could be used for WB, IHC, Flow Cytometry and so on.

Specifications

Product Name

CD99 Mouse mAb - 1차 항체, specific to CD99, Mouse IgG1

동의어

12E7 | E2 antigen | Protein MIC2 | T-cell surface glycoprotein E2

사양 및 순도

ExactAb™, Validated, Carrier Free, 1.0mg/mL

생화학 및 생리적 메커니즘

Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. Post-translational: Extensively O-glycosylated.

숙주 종

마우스

특이성

CD99

면역원

Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.

포지티브 제어

WB: MOLT-4, Jurkat lysates. IHC: Human bladder cancer, ewing sarcoma tissues. Flow Cytometry: Jurkat cells.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

Mouse IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

1.0mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - Western Blot
All lanes: CD99 Mouse mAb (Ab006798) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 19 kDa
Observed band size: 28&32 kDa

Flow Cytometry: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD99 Mouse mAb (Ab006798) (green) and negative control (red).

IHC: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD99 mouse mAb (Ab006798) with DAB staining.

IHC: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human ewing sarcoma tissues using CD99 (Ab006798) mouse mAb with DAB staining.

적용

• 적용: Dilution info
• WB:

  1/500-1/2000;

• ELISA:

  1/10000 ;

• IHC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

관련 대상(인간)

CD99 Tbio CD99 antigen (0 Activities)

Discover Target: CD99

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)