GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Store at -80°C,Avoid repeated freezing and thawing
배송
Dry ice packs + Cold packs
안정성 및 스토리지
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
CDA Mouse mAb (Ab095561) - Western Blot All lanes: CDA Mouse mAb (Ab095561) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 15.0 s
CDA Mouse mAb (Ab095561) - IHC IHC-P was performed using sections of the formalin-fixed paraffin-embedded spleen tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with CDA Mouse mAb (Ab095561) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cells in red pulp are positively stained.
CDA Mouse mAb (Ab095561) - IP Immunoprecipitation was performed by incubation of 2.5 µg CDA Mouse mAb (Ab095561) with SK-OV-3 lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CDA Mouse mAb (Ab095561) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: SK-OV-3 lysate Lane 2: CDA immunoprecipitated from SK-OV-3 lysate by CDA Mouse mAb (Ab095561) Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody Result: CDA Mouse mAb (Ab095561) can immunoprecipitate CDA.
적용
적용
Dilution info
WB
1/1000
IP
1/100
IHC
1/400
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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