The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP). 3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.
Application:
DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
In situ hybridization
Western blot
Dot blot
DAB substrate has been used in immunofluorescence and western blot.
사양
Product Name
DAB Substrate
사양 및 순도
dark brown, /black precipitate; Visually evaluated
분자 유형
Bioreagents/Buffer
보관 및 배송
집중력
dark brown, /black precipitate; Visually evaluated
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