Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The accuracy of enzyme-coupled colorimetric reactions depends on whether the roles of auxiliary enzymes are clearly defined. Mutarotase accelerates the equilibrium of sugar anomers. Oxidases and dehydrogenases convert analytes into intermediate signals such as H₂O₂ or NAD(P)H. Peroxidases ...
The creatininase method is a commonly used quantitative method for creatinine determination in clinical biochemistry and scientific research. It mainly converts creatinine into a measurable colorimetric signal through an enzymatic cascade reaction. Compared with the alkaline picrate method, the ...
Enzymatic protein crosslinking can regulate the interconnection mode among protein molecules under mild conditions and is an important method for the construction of functional gels. This system is applicable to the development of food gels, biomedical hydrogels, active substance delivery ...
Horseradish peroxidase (HRP) is a heme-dependent peroxidase isolated from the root tissue of horseradish (Armoracia rusticana) and belongs to the oxidoreductase family. HRP uses hydrogen peroxide (H₂O₂) or organic peroxides as oxidants to catalyze the oxidation of a wide variety of aromatic ...
Horseradish Peroxidase (HRP) is an enzyme widely used in biochemical experiments. With its ability to catalyze the decomposition of hydrogen peroxide, HRP generates detectable products through oxidation-reduction reactions involving substrates.
Techniques often explored alongside immunological experiments.
