dUTP (100mM) - BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM , CAS No.102814-08-4

CAS: 102814-08-4 Cat. No.: D745475 분자식: C₉H₁₂N₂Na₃O₁₄P₃ 분자량: 534.09

GRADE & PURITY BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, Sterile, for DNA and RNA applications, ≥99%, 100mM

Synonyms

DUTP | 2'-deoxyuridine 5'-triphosphate | 2'-Deoxyuridine-5'-triphosphate, sodium salt solution | 2'-deoxyuridine triphosphate | deoxy-UTP | uridine triphosphate | 2'-Deoxyuridine 5'-triphosphate solution | 2'-Deoxyuridine-5'-Triphosphoric Acid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

개요

dUTP, 2'-deoxyuridine 5'-triphosphate, is commonly used in PCR with Taq DNA polymerase, primer extension, and cDNA synthesis. It is commonly used in conjunction with the UDG enzyme that can recognize and excise deoxyuracil bases to prevent carryover contamination by PCR products from surroundings. dUTP can be used in PCR with polymerase family A members such as Taq, and also for isothermal amplification with Bst DNA Polymerase. Some enzymes that can use dATP, dTTP, dGTP, dCTP, may not be able to use dUTP. Please refer to manufacture's instructions of a particular enzyme or make a test.In PCR with Taq DNA polymerase, dUTP can replace dTTP or can be added to the reaction mix at an equal concentration to other dNTPs. For isothermal amplification with Bst DNA Polymerase, dUTP can replaced 50% dTTP at most. Otherwise, the isothermal amplification will be significantly inhibited.Usually, dUTP and uracil DNA glycosylase (UDG enzyme) are used in conjunction to establish the dUTP/UDG anti-pollution system in which the UDG enzyme can enzymatically remove possible contamination from previous PCR products containing deoxyuracil (dU), thus effectively minimize cross-over contamination.The molecular formula of dUTP is C9H12N2O14P3Na3 with a molecular weight of 534.1 (468.1 in acid form). Its maximum absorption wavelength is 262nm.This dUTP solution is prepared with ultrapure water to a concentration of 100mM. The pH is titrated to 7.0-7.5 with high-purity NaOH solution.This product is free of DNase and RNase, and can be directly used for different molecular biology applications.

Precautions：

The UDG enzyme does not work for cases that have been contaminated by dU-free PCR products. In this case, the primers can be redesigned to amplify other regions of target DNA. The dUTP/UDG anti-pollution system should be established to minimize cross-over contamination.This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

None

Specifications

동의어

DUTP | 2'-deoxyuridine 5'-triphosphate | 2'-Deoxyuridine-5'-triphosphate, sodium salt solution | 2'-deoxyuridine triphosphate | deoxy-UTP | uridine triphosphate | 2'-Deoxyuridine 5'-triphosphate solution | 2'-Deoxyuridine-5'-Triphosphoric Acid

사양 및 순도

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM

안정성 및 스토리지

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

이 제품은 콜드 체인 배송이 필요합니다.지상 및 기타 경제 서비스는 사용할 수 없습니다.

등급

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology, 불임

순수함

≥99%

이름과 식별자

PH

7.0-7.5

분자량

534.09

자주 묻는 질문

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does DNase, RNase free mean?

DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 102814-08-4, the molecular formula is C9H12N2Na3O14P3, and the molecular weight is 534.09 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.