등급 & 순도Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
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보관 및 배송
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
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품질 문서
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
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문헌적 근거
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
개요
Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on.
Carrier Free, ExactAb™, Validated, High Performance, See COA
생화학 및 생리적 메커니즘
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
숙주 종
마우스
특이성
EGFR
면역원
Recombinant EGFR expressed in E.coli (AA 888-1210)
포지티브 제어
WB: A431, HeLa, MCF7, A549, NIH/3T3 and HUVEC cell lysates. IHC: Human kidney, placent, lung cancer, stomach, adrenal gland, liver cancer tissues. IF/ICC: HeLa cells.
변화
Unconjugated
등급
Carrier Free, ExactAb™, High Performance, Validated
아이소타입
IgG2b
라이트 체인 유형
kappa
SDS-PAGE
150 kDa
정화 방법
Protein A + Protein G purified
집중력
See COA
Product Line
생물 제품
보관 및 배송
모양
Liquid
보관 조건
Store at -20°C,Avoid repeated freezing and thawing
배송
Ice chest + Ice pads
안정성 및 스토리지
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
이미지
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 10.4 s
EGFR Mouse mAb (Ab101163) - Western Blot All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 175 kDa Observed band size: 169 & 69 kDa Exposure time: 2.3 s
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Lung cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Stomach Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Adrenal gland Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
EGFR Mouse mAb (Ab101163) - IF/ICC IF analysis of EGFR Mouse mAb (Ab101163) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with EGFR Mouse mAb (Ab101163) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
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