Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA Azide Free,Carrier Free,Low Endotoxin,PBS Only,Validated 최소한의 기준선 간섭이 필요한 민감한 크로마토그래피 및 분석 워크플로우용.
Store at -20°C,Avoid repeated freezing and thawing Ice chest + Ice pads 출고. 정확한 사양은 로트별 COA를 확인하세요.
SDS, COA, 데이터시트, 사양서를 다운로드할 수 있습니다. 로트별 COA는 로트 번호 조회를 통해 확인할 수 있습니다.
크로마토그래피, 유기 합성 및 교차 커플링 반응 분야의 동료 심사 논문 0편에서 인용되었습니다.
Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for WB, IHC, ICC/IF, IP, Flow, Neutralization, IP-Mass Spectrometry and so on.
Application
ICC/IF: 5 µg/mL
IP: 2 µg/mL
Flow: 1-5 ug/mL
IHC: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Neutralization: Use at an assay dependent concentration.
IP-Mass Spectrometry: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
EGFR Mouse mAb (Ab101156) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101156) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
5.0 μg/mL
Use at an assay dependent concentration.
Use at an assay dependent concentration.
2 µg/mL
1-5 μg/mL
Use at an assay dependent concentration.
Use at an assay dependent concentration.
위험성, 취급, 보관 및 규제 준수를 포괄하는 종합 문서.
<<<<<<< HEAD SDS 다운로드 → ======= SDS 다운로드 → >>>>>>> release248-p3| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | 날짜 | 항목 |
|---|---|---|---|
 ZJ24F0707957 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
| ZJ24F0707958 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
| ZJ24F0707959 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
당사의 등급 선택 가이드는 카탈로그의 모든 변형 제품에 대해 순도, 안정제 상태 및 용도 적합성을 다룹니다.
Carrier Free 등급 가이드 보기 → Validated 등급 가이드 보기 → Low Endotoxin 등급 가이드 보기 → Azide Free 등급 가이드 보기 → PBS Only 등급 가이드 보기 →
