Elastase, CAS No.39445-21-1

CAS: 39445-21-1 Cat. No.: np001097 EC 번호: 254-453-6 PubChem CID: 363902459

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

Accession #

P00772

Bioactivity

≥50 U/mg protein

개요

Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.

Biochemical Physiological 

Actions Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Form: 2X crystallized. Aqueous suspension containing 0.01% NaN₃ as preservative. 

Greater that 50 units per mg of protein on the substrate elastin-porcein. One unit will solubilize 1 mg of elastin in 20 minutes at pH 8.8 and 37°C.

Greater that 4 units per mg of protein on the substrate Suc-(Al)₃-pNA. One will hydrolyze 1 µ mole per minute at pH 8.3 and 25°C. 

Preparation: Two times crystallized from the euglobin fraction of porcine pancreas. Does not contain trypsin or chymotrypsin. 

Description: Hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. 

Inhibitors: DFP, elastinal, α-2-macroglobulin.

Specifications

Product Name

Elastase, CAS No.39445-21-1

동의어

pancreatic elastase | pancreatic elastase I | pancreatopeptidase E | Elastase from hog pancreas | CELA1 | ELA1 | ELS1 | Elastase-1

등급

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

사양 및 순도

Bioactive,ActiBioPure™,Native,High Performance,EnzymoPure™,from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

생화학 및 생리적 메커니즘

Serine proteases that hydrolyze many proteins in addition to elastin.

생체 활성

≥50 U/mg protein

가입 번호

P00772

예상 분자량

25.5 kDa

CAS

39445-21-1

효소 커미션 번호

EC 3.4.21.36

분자 유형

Enzyme

보관 및 배송

집중력

from Porcine pancreas; ≥50 U/mg protein; Protein concentration: See COA; 2× Crystallized

보관 조건

Store at 2-8°C

배송

Wet ice

안정성 및 스토리지

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

단위 정의

One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8.

참고 문헌

1. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao.  (2024)  Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo.  INDUSTRIAL CROPS AND PRODUCTS,      [PMID:] [10.1016/j.indcrop.2024.118716]