This method is suitable for pure culture and preservation of strains, and can also be used for identification tests of urea and Kirschner's bisaccharides media. The method of inoculation varies slightly depending on the purpose. Inoculation method for pure culture is to sterilize the inoculation ring (needle), pick a single colony from the bottom of the medium up a straight line, and then serpentine line inoculation in agar slant can be used to identify the culture medium slant inoculation is to use the inoculation needle, pick the bacteria to be identified colonies, generally should be taken to the top of the colony, the first use of the inoculation needle inserted into the middle of the slant vertically stabbed into the bottom, and then extracted in the slant for the serpentine line can be made. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for testing majors.
Operation method
Bacterial isolation by inoculation with slanting medium method
Principle
This method is suitable for pure culture and preservation of strains, and can also be used for identification tests of urea and Kirschner's bisaccharides media. The method of inoculation varies slightly depending on the purpose. Inoculation method for pure culture slant is the inoculation ring (needle) sterilization, pick a single colony from the bottom of the medium up a straight line, and then serpentine line inoculation in agar slant can be used to identify the culture medium slant inoculation is to use the inoculation needle, pick the bacteria to be identified colonies, generally should be taken to the top of the colony, the first use of the inoculation needle inserted into the middle of the slant vertically stabbed the bottom, and then extracted in the slant on the snake can be used to draw a line. Move For more product details, please visit Aladdin Scientific website.