Manidipine 2HCl: Properties, Uses & Lab FAQs

Manidipine 2HCl is a dihydropyridine-class L-type calcium channel blocker supplied as the dihydrochloride salt for improved aqueous solubility, widely used in cardiovascular pharmacology research.

Chemical Class

Dihydropyridine calcium channel blocker (1,4-DHP)

Salt Form

Dihydrochloride (2HCl) for enhanced solubility

Primary Target

Voltage-gated L-type calcium channels (Cav1 family)

Research Application

Antihypertensive mechanism studies, vascular smooth muscle research

SKU

M129521

Recommended Storage

Store at -20 °C, protected from light and moisture

What Is Manidipine 2HCl?

Manidipine 2HCl is the dihydrochloride salt of Manidipine, a third-generation 1,4-dihydropyridine (DHP) calcium channel antagonist. By blocking voltage-gated L-type (Cav1) calcium channels in vascular smooth muscle cells, Manidipine reduces intracellular calcium influx, leading to vasodilation and lowered peripheral resistance. The dihydrochloride salt form (2HCl) is specifically selected for research use because it confers greater aqueous solubility compared with the free base, facilitating preparation of stock solutions for in vitro assays.

In the research setting, Manidipine 2HCl is employed as a pharmacological tool to dissect calcium-dependent signalling pathways, study vascular tone regulation, and evaluate cardioprotective or renoprotective mechanisms at the cellular and molecular level.

Mechanism of Action

Manidipine acts selectively on L-type voltage-operated calcium channels (VOCCs), which are the primary route for calcium entry in vascular smooth muscle and cardiac cells. Binding occurs at the DHP receptor site on the α1 subunit of the channel, stabilising the channel in its inactivated (closed) state and thereby reducing calcium influx in a concentration-dependent manner.

Compared with earlier DHP compounds, Manidipine exhibits high lipophilicity (before salt conversion), which contributes to a long duration of action and gradual onset — properties that are relevant when designing prolonged in vitro exposure experiments. Researchers should account for the 2HCl salt correction factor when calculating molar concentrations from mass.

Solubility, Preparation & Storage

Solubility: The 2HCl salt form of Manidipine is soluble in water and aqueous buffers to a greater extent than the free base. DMSO is also a suitable solvent for preparing concentrated stock solutions. Always verify solubility empirically at your working concentration before use in sensitive assays.

Stock solution preparation: Dissolve the powder completely in DMSO or aqueous vehicle, vortex thoroughly, and warm gently if necessary. For cell-based assays, dilute DMSO stocks to keep final DMSO concentration ≤0.1% to avoid vehicle effects. Use the molecular weight of the 2HCl salt (not the free base) for accurate molar calculations.

Storage: Store lyophilised or powdered Manidipine 2HCl at −20 °C in a tightly sealed container, protected from light and moisture. Prepared stock solutions should be aliquoted and stored at −20 °C; avoid repeated freeze–thaw cycles. Stability data for specific lots should be confirmed with the Certificate of Analysis (CoA).

Common Research Applications

Manidipine 2HCl is used across a range of in vitro and in vivo research contexts:

• Vascular pharmacology: Investigating vasodilatory responses and vascular smooth muscle calcium signalling.
• Renal research: Studying renoprotective effects associated with calcium channel blockade, including glomerular haemodynamics.
• Cardioprotection studies: Evaluating effects on cardiomyocyte calcium handling and ischaemia-reperfusion models.
• Hypertension models: Using Manidipine 2HCl as a reference antihypertensive compound in rodent or cell-based models.
• Signal transduction: Probing the role of L-type calcium channels in downstream signalling cascades such as calmodulin/CaMKII and NFAT pathways.

Researchers should validate compound activity in their specific model system and consult the latest literature for appropriate concentration ranges.

Quality, Purity & Ordering Information

Aladdin Scientific supplies Manidipine 2HCl (SKU: M129521) as a research-grade reagent. Each lot is accompanied by a Certificate of Analysis (CoA) documenting identity (typically confirmed by NMR and/or HPLC), purity, and appearance. Researchers requiring specific purity thresholds or additional characterisation data (e.g., elemental analysis, mass spectrometry) should contact Aladdin Scientific technical support or consult the product page for the current lot CoA.

For bulk quantities, custom packaging, or regulatory documentation (e.g., for GMP-adjacent research), please contact the Aladdin Scientific sales team directly. Volume pricing and institutional purchasing arrangements may be available.

Frequently asked questions

What is the difference between Manidipine free base and Manidipine 2HCl?

Manidipine 2HCl is the dihydrochloride salt of Manidipine, formed by protonation of two basic nitrogen atoms with hydrochloric acid. The salt form has a higher molecular weight than the free base and significantly improved aqueous solubility, making it more convenient for preparing stock solutions in water or aqueous buffers. When calculating molar concentrations, always use the molecular weight corresponding to the 2HCl salt form.

How should I prepare a stock solution of Manidipine 2HCl for cell-based assays?

Dissolve Manidipine 2HCl in DMSO or sterile water to prepare a concentrated stock solution (e.g., 10–50 mM), vortex until fully dissolved, and aliquot to avoid freeze–thaw cycles. For cell-based assays, dilute the stock in culture medium immediately before use, ensuring the final DMSO concentration does not exceed 0.1% to minimise solvent cytotoxicity. Include a vehicle-only control in your experimental design.

What storage conditions are recommended for Manidipine 2HCl?

Store Manidipine 2HCl powder at −20 °C in a sealed, light-protected container away from moisture. Prepared aliquots of stock solutions should also be kept at −20 °C and used within a validated stability window. Refer to the Certificate of Analysis (CoA) supplied with your specific lot for confirmed stability data.

Which calcium channel subtype does Manidipine 2HCl primarily target?

Manidipine 2HCl primarily targets voltage-gated L-type calcium channels (Cav1 subfamily), acting at the 1,4-dihydropyridine (DHP) binding site on the channel's α1 subunit. L-type channels are the predominant calcium entry pathway in vascular smooth muscle cells and cardiomyocytes, making this compound a valuable tool for studying calcium-dependent processes in cardiovascular tissues.

Is Manidipine 2HCl suitable for in vivo animal studies?

Manidipine 2HCl is supplied as a research reagent and may be used in in vivo pharmacology studies; however, researchers are responsible for determining appropriate dosing, vehicle formulation, and ethical compliance for their specific animal models. The improved aqueous solubility of the 2HCl salt can facilitate formulation for parenteral or oral administration in preclinical models. Always consult current literature for reported dose ranges and consult your institutional animal ethics committee.

References

1. Leonetti G, Cuspidi C. Choosing the right ACE inhibitor or calcium antagonist in hypertension: a focus on Manidipine. Drugs. (General reference to dihydropyridine CCB class pharmacology — specific article details require verification.)
2. Triggle DJ. 1,4-Dihydropyridines as calcium channel ligands and privileged structures. Cell Mol Neurobiol. (Textbook-level reference for DHP mechanism — specific volume/page require verification.)
3. Aladdin Scientific. Manidipine 2HCl Product Page, SKU M129521. Aladdin Scientific catalogue (accessed 2024).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.