PIP4K2A Mouse mAb: FAQs for Research Use

PIP4K2A Mouse mAb is a monoclonal antibody raised in mouse targeting PIP4K2A (phosphatidylinositol-5-phosphate 4-kinase type-2 alpha), a key enzyme in phosphoinositide signaling pathways relevant to cell growth, stress response, and metabolic regulation.

Product Name

PIP4K2A Mouse mAb

SKU

Ab350631

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS only (no carrier protein, no preservative)

Target Protein

PIP4K2A (Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha)

Supplier Reference

absea #KC-22638

What Is PIP4K2A and Why Is It a Research Target?

PIP4K2A (Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha, also abbreviated PI5P4Kα) is a lipid kinase that phosphorylates phosphatidylinositol-5-phosphate (PI5P) at the D-4 position to generate phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. This enzyme belongs to the type II phosphatidylinositol phosphate kinase (PIPKII) family and is encoded by the PIP4K2A gene.

PIP4K2A plays regulatory roles in multiple cellular processes including mTORC1 signaling, autophagy, cellular stress responses, and metabolic homeostasis. It has attracted growing interest as a potential therapeutic target in oncology and metabolic disease research. Researchers use anti-PIP4K2A antibodies to study its expression levels, subcellular localization, and protein–protein interactions across a variety of cell lines and tissue models.

Product Specifications and Formulation Details

The Aladdin Scientific PIP4K2A Mouse mAb (SKU: Ab350631) is a monoclonal antibody of mouse origin supplied in phosphate-buffered saline (PBS) only. The formulation contains no added carrier proteins (such as BSA) and no preservatives (such as sodium azide), which can be advantageous for downstream conjugation, functional assays, or applications sensitive to carrier protein interference.

This product is referenced under the supplier code absea #KC-22638. Researchers requiring specific details such as exact concentration, isotype, clone number, immunogen sequence, or lot-specific quality-control data should consult the product datasheet available on the Aladdin Scientific website or contact technical support directly, as these parameters may vary by lot.

Recommended Applications and Usage Guidelines

Anti-PIP4K2A monoclonal antibodies are typically employed in standard immunological and biochemical assays. Common applications for antibodies of this class include Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). However, validated applications for Ab350631 specifically should be confirmed on the product datasheet, as suitability for each technique depends on the clone's epitope and affinity characteristics.

Users should perform optimization experiments to determine the appropriate working dilution for each application and sample type. For Western blotting, it is advisable to include a positive control lysate from a cell line known to express PIP4K2A. All handling should follow standard good laboratory practice (GLP) for antibody-based reagents.

Storage, Handling, and Stability

As this antibody is formulated in PBS only (without stabilizing additives such as glycerol or BSA), proper storage conditions are especially important to maintain activity and prevent degradation. General best practices for monoclonal antibodies in PBS include:

• Store at –20 °C for long-term storage; avoid repeated freeze–thaw cycles by preparing single-use aliquots upon receipt.
• For short-term use (up to 1–2 weeks), the antibody may be stored at 4 °C.
• Do not store at room temperature for extended periods.
• Keep away from light if fluorescent conjugates are used in downstream steps.

Always refer to the lot-specific datasheet for validated storage recommendations. If the datasheet specifies conditions different from the above general guidance, the datasheet takes precedence.

Ordering, Availability, and Technical Support

PIP4K2A Mouse mAb (Ab350631) is available for order through the Aladdin Scientific website or via your regional Aladdin Scientific distributor. When placing an order, reference SKU Ab350631 to ensure correct product selection.

For questions regarding pricing, volume discounts, custom conjugation services, or bulk procurement, please contact the Aladdin Scientific sales team. Technical inquiries regarding antibody performance, protocol optimization, or troubleshooting can be directed to Aladdin Scientific technical support. Typical information to have on hand when contacting support includes the lot number, application, species/sample type, and observed results.

Returns, refunds, and shipping policies are governed by Aladdin Scientific's standard terms; please consult the relevant policy pages on the Aladdin Scientific website or contact customer service for details.

Frequently asked questions

What is PIP4K2A Mouse mAb (Ab350631) and what does it target?

PIP4K2A Mouse mAb (Ab350631) is a mouse monoclonal antibody designed to detect PIP4K2A, the alpha isoform of type II phosphatidylinositol-5-phosphate 4-kinase, a lipid kinase involved in phosphoinositide metabolism and cellular signaling. It is referenced under supplier code absea #KC-22638.

What applications is Ab350631 validated for, and what species does it react with?

Validated applications and species reactivity for Ab350631 are specified on the lot-specific product datasheet. While antibodies targeting PIP4K2A are commonly used in Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry, users should confirm which applications have been experimentally validated for this specific clone by consulting the product datasheet or contacting Aladdin Scientific technical support.

Why is Ab350631 formulated in PBS only, and how does this affect my experiments?

The PBS-only formulation means the antibody contains no carrier proteins (e.g., BSA) or preservatives (e.g., sodium azide), making it well-suited for applications where these additives could interfere, such as direct conjugation to labels or enzymes, and assays sensitive to azide toxicity. However, without stabilizing additives, careful aliquoting and storage at –20 °C are essential to preserve antibody activity.

How should I store PIP4K2A Mouse mAb to maintain its performance?

For long-term storage, keep Ab350631 at –20 °C in single-use aliquots to avoid repeated freeze–thaw cycles, which can reduce antibody activity. For short-term use within one to two weeks, storage at 4 °C is acceptable. Always refer to the lot-specific datasheet for the manufacturer's validated storage guidance.

How do I place an order for Ab350631, and what information should I have ready?

You can order Ab350631 directly through the Aladdin Scientific website or via your regional distributor by quoting SKU Ab350631. For technical or purchasing inquiries, having the following information ready will expedite assistance: the desired quantity, your institution's shipping and billing details, and any specific formulation or certification requirements.

References

1. Emerling BM et al. Depletion of a putatively druggable class of phosphatidylinositol kinases inhibits growth of p53-null tumors. Cell. 2013;155(4):844-857. doi:10.1016/j.cell.2013.09.057
2. Lamia KA et al. Compromised intestinal epithelial barrier in mice lacking phosphatidylinositol-5-phosphate 4-kinase alpha. Proceedings of the National Academy of Sciences. 2004;101(31):11123-11128. doi:10.1073/pnas.0404601101
3. Aladdin Scientific product datasheet: PIP4K2A Mouse mAb, SKU Ab350631 (absea #KC-22638).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.