Experiments to determine the effect of biological factors on microorganisms
The antimicrobial spectrum of different antibiotics is different, some antibiotics only have antibacterial effects on a few bacteria, polymyxin only has an effe
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Yeast cell plasmid isolation experiment
Under non-selective growth conditions, most E. coli/yeast shuttle vectors are lost at a frequency of about 1%, and one plasmid that is not easily isolated and r
Yeast cell nucleus preparation experiment
The nucleus (nucleus) is the largest and most important organelle in the cell (the old junior high school textbooks believe that the nucleus is not an organelle
Handling experiments on pre-coated slides
Slides coated with polylysine (a Thermo product) with durable bioadhesion for attaching frozen and paraffin-embedded sections that can be subsequently centrifug
Yeast vector and expression product detection experiments
Because of the simplicity and sensitivity of the β-galactosidase assay, yeast genes are frequently labeled with the functional portion of the lacZ gene to indi
Yeast protoplast preparation experiment
Protoplasts are formed by the differentiation of protoplasm, specifically the cell membrane and intramembranous cytoplasm and other life-active organelles. In p
Frozen section fixation experiment
This experiment is mainly used for fixing frozen sections.
Yeast gene cloning experiment
A general method for cloning yeast genes by complementation using a putative mutation. The mutation is cdc101-1, which is both recessive and temperature sensiti
Tissue and embryo fixation and embedding experiments
The role of fixation, from the histological point of view its simple definition is to maintain the intrinsic morphology and structure of cells and tissues, so t