Chromosome preparation experiments on cultured peripheral blood cells
Although chromosomes can be prepared from other cells, human peripheral blood leukocytes are the easiest to synchronize, allowing high-resolution analysis of th
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Cytoplasmic RNA extraction
Since there are many sources of RNA species, there are different methods of extraction and preparation, generally phenol method, detergent method and guanidine
Poly(A)+ RNA Preparation Experiments
The use of cND A microarray technology can be used to (1) analyze and detect the expression of thousands of genes in parallel; (2) play an important role in the
In vitro splicing analysis experiments in mammalian cells
It is typical for splicing reactions to use nuclear extracts, i.e., S100 extracts complementing partially purified fractions of SR proteins or crude extracts, m
Fungal RNA extraction
Fungal RNA extraction can be: (1) used in the study of fungal RNA interference mechanism; (2) used in cDNA library construction and other studies; (3) used in t
Experiments on the isolation and analysis of pre-splitters and spliceosomes
The spliceosome is a ribonucleoprotein body (RNP) composed of RNA and proteins, which removes introns from precursor mRNA during the splicing process of precurs
Yeast Spliceosome Analysis Experiment
A precursor is a macromolecular ribonucleoprotein complex that splices mRNA precursors in the nucleus of eukaryotic cells. It consists of five snRNPs and a larg
RNA extraction and labeling experiments
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cDNA synthesis technology
After the mRNA of the target gene has been identified by in vitro cell-free translation, the first strand of cDNA can be synthesized in the steps described in t