Northern Blotting Technology
Blot hybridization is the process of binding a nucleic acid fragment to be tested to a certain solid phase support and then hybridizing it with a labeled probe
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Detection of RNA-RNA binding protein interaction assay using E. coli
Binding of heterologous RNA-BPs to target RNAs in E. coli inhibits translation of target genes. This method has been successfully applied to the study of a vari
Psoralen-mediated photocrosslinking reaction to study the interaction between RNA and protein molecules experimentally
An RNA-protein molecular complex analysis method based on the photochemical reaction of psoralen enables the analysis of the topological conformation of RNA-pro
Gene cloning: efficient receptor cell production
Highly efficient receptor cells can be used for plasmid transformation.DNA cloning refers to the process of molecular manipulation in which a DNA fragment conta
denaturing gradient gel electrophoresis
Denaturing gradient gel electrophoresis (DGGE) is a gel system that enables the separation of DNA fragments according to their melting properties. (1) By settin
Subcloning of target genes
Subcloning of the target gene can be applied to (1) further analysis of the target gene and (2) gene recombination.
Plasmid preparation
Plasmid preparation can be used for (1) carrying exogenous genes into bacteria for amplification or expression of the main vector, which has an important role i
In Situ Hybridization Requirements and Procedures
In situ hybridization can be used for (1) solid-phase molecular hybridization and (2) labeled DNA or RNA as probes to detect specific nucleic acid sequences in
Determination of the amount of DNA fixed in ethanol
Determination of DNA content can be used to (1) perform gene fragment ligation experiments. (2) Perform plasmid concentration determination prior to transformat