Dephosphorylation experiments on enzyme section breaks
In the modification of DNA fragments, the dephosphorylation reaction is an important element, which is catalyzed by alkaline phosphatase, an enzyme that removes
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Experimental double deoxy sequencing reaction with T7 phage DNA polymerase
This protocol is modeled after the method of Tabor and Richardson (1987a, 1989b, 1990), which uses a single-stranded DNA template for DNA sequencing (for single
Experiments on the preparation of denaturing templates for sequencing by double deoxy chain termination method
The following method of denaturing plasmid DNA by base denaturation should be used in conjunction with Scheme 3, as sequencing enzymes are used to catalyze the
Dideoxy sequencing reaction experiment with Taq DNA polymerase
In addition to its use in PCR reactions, heat-stabilized DNA polymerase can be used as a replacement for sequencing enzymes or Klenow fragments for double deoxy
Double deoxygenation sequencing using E. coli DNA polymerase I Klenow fragments and single-stranded DNA templates
When Sanger and colleagues established the first DNA strand termination extension method, there was only one suitable DNA polymerase available, the Klenow fragm
Inoue method experiments for the preparation and transformation of susceptible Escherichia coli (preparation of super-susceptible cells)
Inoue's method (1990) for the preparation of E. coli receptor cells is good enough to achieve even the transformation efficiency of Hanahan's method (1980). How
Radiation autoradiography and DNA sequence reading experiments from sequencing gels
This experiment describes the procedure regarding radioactive autoradiography and the reading of DNA sequences from sequencing gels. This experiment comes from
Experiments on the preparation of denaturing polyacrylamide gels
In sequencing, 4 series of DNA fragments are analyzed by electrophoresis on a thin polyacrylamide gel under denaturing conditions. This protocol describes the p
Experiments on the preparation of polyacrylamide gels containing formamide
A common problem in DNA sequencing is the compression of electrophoretic bands due to the presence of DNA dipolar structures. A simple and straightforward solut