Determination of β-galactosidase in cellular extracts of experimental
This protocol describes the determination of expressed β-galactosidase activity after transfection of mammalian cells with the reported molecular vector (see F
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M13 Phage spread plates
M13 Phage plaques are formed when a single virus infects a single bacterium. The daughter virus particles infect neighboring bacteria and then produce the next
Large-scale preparation of single- and double-stranded M13 phage DNAs
This protocol is mainly used to prepare large quantities of double-stranded DNA from M13 phage, which is often used as a cloning vector in the laboratory, as we
M13 Preparation of phage double-stranded (replicative) DNA
Bacteria infected with M13 phage contain viral double-stranded RF DNA, and single-stranded daughter DNA is contained in crude viral particles in the culture med
M13 Preparation of phage single-stranded DNA
M13 phage single-stranded DNA was prepared from viral particles secreted by infected cells into the surrounding culture medium. filoviruses were first concentra
M13 Phage liquid culture
M13 Phage progenitors are usually grown in liquid culture, where infected cells do not lyse but grow slowly to form a dilute suspension. Inoculation is almost a
P1 Application of phages and their cloning systems
The P1 phage was discovered in the same year as the λ phage (Bertani 1951). Both phages are mild phages in their natural hosts, and their research history in t
M13 Cloning of phage vectors
Although there is theoretically no limit to the number of fragments of exogenous DNA that can be carried by a recombinant M13 phage, in practice there is a limi
Ligation experiments of λ phage arms to exogenous genomic DNA fragments
When a λ phage arm is attached to an exogenous genomic DNA fragment, two parameters must be taken into account: the molar ratio of the phage arm to the potenti