Quantitative analysis of trace DNA by PCR method
PCR is used to quantify the amount of a particular DNA sequence from 1 to 20,000 molecules per sample. In addition, it can assist in assessing the presence of c
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
DNA barcode collection-analysis protocol experiment
The rise of biological barcoding demonstrates the active search for a system to identify eukaryotes based on the analysis of sequence diversity in short, standa
Synthesis of radiolabeled minus cDNA probes using oligo (dT) as primer
This protocol describes the preparation of a deduction cDNA probe by hybridization with an mRNA driver followed by purification of single-stranded radiolabeled
DNA electrophoresis (agarose gel)
DNA electrophoresis can be used to (1) separate DNA fragments of different sizes, (2) identify target DNA fragments, and (3) purify and recover DNA fragments.
Poxvirus DNA extraction experiment
If extracted poxvirus DNA is used for transfection, it should be isolated after proteinase K digestion and phenol extraction. See this experiment for specific m
Automated Laser Fluorescence DNA Sequencing
At present, the application of automated laser fluorescence DNA sequencing technology (also known as first generation sequencing technology) has been very commo
Pyrophosphate sequencing
Pyrosequencing is a luminescence-based sequencing technology for the determination of pyrophosphate (PPi). The three key points of pyrosequencing are: (1) dATPa
Mutagenesis of genomes using chemical mutagens
Currently, there are two main strategies for studying gene function using model mice: the gene-driven approach and the phenotype-driven approach. Gene knockout
DNA recombination in vitro
DNA recombination, also known as gene cloning, is the most basic technique in gene manipulation and the core technology of molecular biology.DNA recombination r